Anti-Cytomegalovirus antibody [6F12] (ab6500)
Key features and details
- Mouse monoclonal [6F12] to Cytomegalovirus
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Human cytomegalovirus
- Isotype: IgG1
Overview
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Product name
Anti-Cytomegalovirus antibody [6F12]
See all Cytomegalovirus primary antibodies -
Description
Mouse monoclonal [6F12] to Cytomegalovirus -
Host species
Mouse -
Specificity
Target Protein: Cytomegalovirus ICP22 Delayed Early Protein, also known as HWLF1. -
Tested applications
Suitable for: WB, ELISA, ICC/IFmore details -
Species reactivity
Reacts with: Human cytomegalovirus -
Immunogen
Tissue, cells or virus corresponding to Cytomegalovirus Cytomegalovirus.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
6F12 -
Myeloma
NS1/1-Ag4-1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/100 - 1/800.
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ELISA |
1/102400.
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ICC/IF |
1/800.
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Notes |
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WB
1/100 - 1/800. |
ELISA
1/102400. |
ICC/IF
1/800. |
Target
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Relevance
Cytomegalovirus is a member of the herpes virus group, which includes herpes simplex virus types 1 and 2, varicella zoster virus (which causes chicken pox), and Epstein Barr virus (which causes infectious mononucleosis). These viruses share a characteristic ability to remain dormant within the body over a long period. -
Database links
- SwissProt: P06725 Human cytomegalovirus
- SwissProt: P18139 Human cytomegalovirus
- SwissProt: Q6SW59 Human cytomegalovirus
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Alternative names
- 65 kDa phosphoprotein antibody
- Envelope glycoprotein B antibody
- Human herpesvirus 5 antibody
see all
Images
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See protocol for procedure. Procedure uses 10-well CMV slides and anti-Mouse-FITC F(ab')2 w/ counterstain
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See protocol for procedure. Procedure uses 10-well CMV slides and anti-Mouse-FITC F(ab')2 w/ counterstain
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Determined by IFA (see protocol) at an antibody dilution of 1:100 with anti-Mouse-FITC F(ab')2 w/ counterstain
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See protocol for procedure. Antigen is CMV Infected Cell Extract coated at a dilution of 1:100.
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See protocol for procedure. Antigen is CMV Infected Cell Extract at 10
See protocol for procedure. Antigen is CMV Infected Cell Extract at 10 µg/cm.µ g/cm.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab6500 has been referenced in 1 publication.
- Fukui Y et al. Establishment of a cell-based assay for screening of compounds inhibiting very early events in the cytomegalovirus replication cycle and characterization of a compound identified using the assay. Antimicrob Agents Chemother 52:2420-7 (2008). PubMed: 18458124