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  1. Link

    cytomegalovirus-antibody-ddg9--cch2-prediluted-ab17073.pdf

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Microbiology Organism Virus DNA Virus double stranded DNA Virus CMV
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Anti-Cytomegalovirus antibody [DDG9 & CCH2], prediluted (ab17073)

  • Datasheet
  • SDS
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Key features and details

  • Mouse monoclonal [DDG9 & CCH2] to Cytomegalovirus, prediluted
  • Suitable for: IHC-P, WB, IHC-Fr
  • Reacts with: Human cytomegalovirus
  • Isotype: IgG

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Overview

  • Product name

    Anti-Cytomegalovirus antibody [DDG9 & CCH2], prediluted
    See all Cytomegalovirus primary antibodies
  • Description

    Mouse monoclonal [DDG9 & CCH2] to Cytomegalovirus, prediluted
  • Host species

    Mouse
  • Specificity

    DDG9 is isotype IgG2a, kappa whilst CCH2 is isotype IgG1, kappa.
  • Tested applications

    Suitable for: IHC-P, WB, IHC-Frmore details
  • Species reactivity

    Reacts with: Human cytomegalovirus
  • Immunogen

    Cytomegalovirus-infected cell lysate.

  • Positive control

    • Infected cervix
  • General notes

    This antibody cocktail reacts with an immediate early antigen and an early CMV antigen. DDG9 is isotype IgG2a, kappa whilst CCH2 is isotype IgG1, kappa. The antibodies stain infected cells giving a nuclear staining pattern with an immediate early and an early antigen. In Western blot, DDG9 reacts with 76 kD protein, where as CCH2 reacts with a 43 kD protein.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.3
    Preservative: 0.05% Sodium azide

    Inert stabilizer
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Primary antibody notes

    This antibody cocktail reacts with an immediate early antigen and an early CMV antigen. DDG9 is isotype IgG2a, kappa whilst CCH2 is isotype IgG1, kappa. The antibodies stain infected cells giving a nuclear staining pattern with an immediate early and an early antigen. In Western blot, DDG9 reacts with 76 kD protein, where as CCH2 reacts with a 43 kD protein.
  • Clonality

    Monoclonal
  • Clone number

    DDG9 & CCH2
  • Isotype

    IgG
  • Light chain type

    kappa
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • DNA Virus
    • double stranded DNA Virus
    • CMV

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG - Isotype Control (ab37355)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab17073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration.

No further titration is required Proteolytic treatment is required prior to immunostaining of formalin-fixed paraffin-embedded tissue sections.

WB
Use at an assay dependent concentration. Detects a band of approximately 76 kDa.

DDG9 reacts with 76 kD protein, where as CCH2 reacts with a 43 kD protein.

IHC-Fr
Use at an assay dependent concentration.
Notes
IHC-P
Use at an assay dependent concentration.

No further titration is required Proteolytic treatment is required prior to immunostaining of formalin-fixed paraffin-embedded tissue sections.

WB
Use at an assay dependent concentration. Detects a band of approximately 76 kDa.

DDG9 reacts with 76 kD protein, where as CCH2 reacts with a 43 kD protein.

IHC-Fr
Use at an assay dependent concentration.

Target

  • Relevance

    Cytomegalovirus is a member of the herpes virus group, which includes herpes simplex virus types 1 and 2, varicella zoster virus (which causes chicken pox), and Epstein Barr virus (which causes infectious mononucleosis). These viruses share a characteristic ability to remain dormant within the body over a long period.
  • Database links

    • SwissProt: P06725 Human cytomegalovirus
    • SwissProt: P18139 Human cytomegalovirus
    • SwissProt: Q6SW59 Human cytomegalovirus
    • Alternative names

      • 65 kDa phosphoprotein antibody
      • Envelope glycoprotein B antibody
      • Human herpesvirus 5 antibody
      • Phosphoprotein UL83 antibody
      • UL83 antibody
      see all

    Protocols

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab17073? Please let us know so that we can cite the reference in this datasheet.

    ab17073 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Question

    Has this product been approved for in vitro use?

    Ab17073 (Anti-Cytomegalovirus antibody (DDG9 & CCH2) prediluted

    Read More

    Abcam community

    Verified customer

    Asked on Mar 28 2012

    Answer

    Thank you for contacting us.

    Our products are for research use only and are not intended for diagnostic or therapeutic use. This antibody should be fine for in vitro studies, but has not been approved by any regulatory boards. I hope this helps, please let me know if you need any additional information or assistance.

    Read More

    Abcam Scientific Support

    Answered on Mar 28 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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