Overview

  • Product name
    Anti-Cytomegalovirus Glycoprotein B antibody [2F12]
    See all Cytomegalovirus Glycoprotein B primary antibodies
  • Description
    Mouse monoclonal [2F12] to Cytomegalovirus Glycoprotein B
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Immunogen

    Cytomegalovirus gB Envelpe Protein (UL55, gp58/116, gC-1 and glycoprotein B)

  • Epitope
    Epitope has not been mapped.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    2F12
  • Myeloma
    NS1/1-Ag4-1
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
ICC/IF
  • Application notes
    Immunofluoresence titer 1+ @ 1:12,800, IFA is tested with acetone fixtation.
    ELISA titer 0.100 @ 1:102,400
    Western Blot > 1:4,000
  • Target

    • Relevance
      Human cytomegalovirus (HCMV) glycoprotein B (gB) is an abundant virion envelope protein that has been shown to be essential for the infectivity of HCMV. HCMV gB is also one of the most immunogenic virus-encoded proteins, and a significant fraction of virus neutralizing antibodies are directed at gB.
    • Cellular localization
      Virus envelope.
    • Alternative names
      • CMV glycoprotein B antibody
      • Glycoprotein B antibody
      • HCMV antibody
      • HHV5 glycoprotein B antibody
      • UL55 antibody
      see all

    Images

    • See protocol for procedure. Procedure uses 10-well CMV slides and anti-Mouse-FITC F(ab')2 w/ counterstain.

    • See protocol for procedure. Procedure uses 10-well CMV slides and anti-Mouse-FITC F(ab')2 w/ counterstain.

    • Determined by IFA (see protocol section) at an antibody dilution of 1:100 with anti-Mouse-FITC F(ab')2 w/ counterstain.

    • See protocol for procedure. Antigen is CMV Infected Cell Extract coated at a dilution of 1:100.

    • See protocol for procedure. Antigen is CMV Infected Cell Extract at 10 µg/cm.

      See protocol for procedure. Antigen is CMV Infected Cell Extract at 10 µg/cm.

    References

    This product has been referenced in:
    • Pires F  et al. A rapid magnetic particle-based enzyme immunoassay for human cytomegalovirus glycoprotein B quantification. J Pharm Biomed Anal 156:372-378 (2018). Read more (PubMed: 29758495) »
    • Wu Y  et al. Human cytomegalovirus glycoprotein complex gH/gL/gO uses PDGFR-a as a key for entry. PLoS Pathog 13:e1006281 (2017). Read more (PubMed: 28403202) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    1-5 of 5 Abreviews or Q&A

    Question
    Answer

    Antibody ab54023 has been tested in a neutralization assay that is described in the following reference. Figure 4 shows the data for this clone.

    Kirchmeier M et al. Enveloped virus-like particle expression of human cytomegalovirus glycoprotein B antigen induces antibodies with potent and broad neutralizing activity. Clin Vaccine Immunol 21:174-80 (2014).

    We do not test this antibody in a neutralization assay in-house so I do not have a protocol to send to you. A much simpler assay then the one used in the Kirchmeier paper is briefly described in the following paper. The assay involves counting PFU after incubation of virus with HCMV gB antiserum. The assay is described in the last paragraph of the paper. It does not mention applying this test to this clone or other monoclonal antibodies but it seems like an easy approach.

    EMBO J. 1986 Nov; 5(11): 3057–3063.

    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1167261/?page=1

    The other HCMV antibody you have, ab6499, has not, to our knowledge, been used in a neutralization assay.

    Read More

    Answer

    Thank you for contacting us.

    Unfortunately, the Anti-Cytomegalovirus Glycoprotein B antibody [2F12] (ab6499), this antibody has not as yet been used in staining paraffin embedded sections to our knowledge. We do not have any other antibodies against this target which have been used in this application. However, this does not mean that it would not work, simply that we have not tried it and would therefore not be able to guarantee its performance in this application.

    If you would like to purchase ab6499 and use it with your tissue samples, you would be eligible to participate in the Abtrial scheme. This offer means that if you purchase ab6499 as normal, test the antibody with your samples in IHC-P and let us know of the results through an Abreview (no matter whether positive or negative) you would then be eligible to claim the value of ab6499 off any product in our catalogue. More information on this offer can be found here:

    www.abcam.com/abtrial

    Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.

    If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab6499.

    If ordered tomorrow you can expect to recieve the product by Thursday the 6th of December.

    I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

    Read More

    Answer

    Thank you for contacting us.

    The cells are human fibroblast infected with CMV (AD169) - acetone fixed after 48 hours of infection

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (AD169-infected HFFF)
    Loading amount
    100000 cells
    Specification
    AD169-infected HFFF
    Treatment
    mock/endoH/PNGaseF for 16H
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Superblock Buffer (Pierce) as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C

    Abcam user community

    Verified customer

    Submitted Feb 12 2008

    Answer

    This antibody has never been tested against Rhesus CMV and 64% homology does not sound very promising - you would be better off with a polyclonal antibody. We are in the final stages of characterizing a chicken anti-CMV that may be applicable - will be available in the next month or so. If you need the single specificity (and has access to recombinant gB) perhaps the chicken polyclonal could be made monospecific via immunoaffinity purification.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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