Anti-Cytomegalovirus Glycoprotein B antibody [2F12] (ab6499)
Key features and details
- Mouse monoclonal [2F12] to Cytomegalovirus Glycoprotein B
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Human cytomegalovirus
- Isotype: IgG1
Overview
-
Product name
Anti-Cytomegalovirus Glycoprotein B antibody [2F12]
See all Cytomegalovirus Glycoprotein B primary antibodies -
Description
Mouse monoclonal [2F12] to Cytomegalovirus Glycoprotein B -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISA, ICC/IFmore details -
Species reactivity
Reacts with: Human cytomegalovirus -
Immunogen
Tissue, cells or virus corresponding to Human Cytomegalovirus Glycoprotein B. Human Cytomegalovirus (AD169) infected human fibroblast cells
-
Epitope
Epitope has not been mapped. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
2F12 -
Myeloma
NS1/1-Ag4-1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6499 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) | |
ELISA | ||
ICC/IF |
Notes |
---|
ELISA titer 0.100 @ 1:102,400
Western Blot > 1:4,000
Target
-
Relevance
Human cytomegalovirus (HCMV) glycoprotein B (gB) is an abundant virion envelope protein that has been shown to be essential for the infectivity of HCMV. HCMV gB is also one of the most immunogenic virus-encoded proteins, and a significant fraction of virus neutralizing antibodies are directed at gB. -
Cellular localization
Virus envelope. -
Database links
- Entrez Gene: 3077424 Human cytomegalovirus
- SwissProt: P06473 Human cytomegalovirus
- SwissProt: P13201 Human cytomegalovirus
-
Alternative names
- CMV glycoprotein B antibody
- Glycoprotein B antibody
- HCMV antibody
see all
Images
-
See protocol for procedure. Procedure uses 10-well CMV slides and anti-Mouse-FITC F(ab')2 w/ counterstain.
-
See protocol for procedure. Procedure uses 10-well CMV slides and anti-Mouse-FITC F(ab')2 w/ counterstain.
-
Determined by IFA (see protocol section) at an antibody dilution of 1:100 with anti-Mouse-FITC F(ab')2 w/ counterstain.
-
See protocol for procedure. Antigen is CMV Infected Cell Extract coated at a dilution of 1:100.
-
See protocol for procedure. Antigen is CMV Infected Cell Extract at 10
See protocol for procedure. Antigen is CMV Infected Cell Extract at 10 µg/cm.µ g/cm.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (14)
ab6499 has been referenced in 14 publications.
- Turner DL et al. UL34 Deletion Restricts Human Cytomegalovirus Capsid Formation and Maturation. Int J Mol Sci 23:N/A (2022). PubMed: 35628580
- Flomm FJ et al. Intermittent bulk release of human cytomegalovirus. PLoS Pathog 18:e1010575 (2022). PubMed: 35925870
- Turner DL et al. UL49 is an essential subunit of the viral pre-initiation complex that regulates human cytomegalovirus gene transcription. iScience 25:105168 (2022). PubMed: 36204275
- Turner DL et al. The host exosome pathway underpins biogenesis of the human cytomegalovirus virion. Elife 9:N/A (2020). PubMed: 32910773
- van Senten JR et al. Human Cytomegalovirus-Encoded G Protein-Coupled Receptor UL33 Facilitates Virus Dissemination via the Extracellular and Cell-to-Cell Route. Viruses 12:N/A (2020). PubMed: 32486172