Overview

  • Product name

    Anti-Cytomegalovirus Glycoprotein B antibody [HCMV37]
    See all Cytomegalovirus Glycoprotein B primary antibodies
  • Description

    Mouse monoclonal [HCMV37] to Cytomegalovirus Glycoprotein B
  • Host species

    Mouse
  • Specificity

    This antibody is specific for Cytomegalovirus glycoprotein B late neutralizing antigen. Reacts with MRC-5 infected cells but not uninfected cells.
  • Tested applications

    Suitable for: ELISA, ICC/IF, IHC-Fr, Neutralisingmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Human CMV extracts

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    HCMV37
  • Isotype

    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab54023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
ICC/IF
IHC-Fr
Neutralising
  • Application notes
    ELISA: Use at an assay dependent dilution.
    ICC/IF: Use at an assay dependent dilution (CMV infected cells).
    IHC-Fr: Use at an assay dependent dilution (CMV infected cells).
    Neut: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      Human cytomegalovirus (HCMV) glycoprotein B (gB) is an abundant virion envelope protein that has been shown to be essential for the infectivity of HCMV. HCMV gB is also one of the most immunogenic virus-encoded proteins, and a significant fraction of virus neutralizing antibodies are directed at gB.
    • Cellular localization

      Virus envelope.
    • Alternative names

      • CMV glycoprotein B antibody
      • Glycoprotein B antibody
      • HCMV antibody
      • HHV5 glycoprotein B antibody
      • UL55 antibody
      see all

    Images

    • ab54023, staining Cytomegalovirus Glycoprotein B in monocyte-derived dendritic cells infected with Human cytomegalovirus (VHL/E strain), cultured for 3 hours (left panels) and 24 hours (right panels), by Immunocytochemistry/ Immunofluorescence.

      Cells were fixed with paraformaldehyde and permeabilized with 0.2% Triton X-100 in PBS for 10 minutes at room temperature. Samples were incubated with primary antibody and an AlexaFluor®568-conjugated goat anti-mouse monoclonal IgG was used as the secondary antibody.

    References

    This product has been referenced in:

    • Wang B  et al. Viperin is induced following toll-like receptor 3 (TLR3) ligation and has a virus-responsive function in human trophoblast cells. Placenta 36:667-73 (2015). Read more (PubMed: 25814471) »
    • Kirchmeier M  et al. Enveloped virus-like particle expression of human cytomegalovirus glycoprotein B antigen induces antibodies with potent and broad neutralizing activity. Clin Vaccine Immunol 21:174-80 (2014). Neutralising . Read more (PubMed: 24334684) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Question
    Answer

    Antibody ab54023 has been tested in a neutralization assay that is described in the following reference. Figure 4 shows the data for this clone.

    Kirchmeier M et al. Enveloped virus-like particle expression of human cytomegalovirus glycoprotein B antigen induces antibodies with potent and broad neutralizing activity. Clin Vaccine Immunol 21:174-80 (2014).

    We do not test this antibody in a neutralization assay in-house so I do not have a protocol to send to you. A much simpler assay then the one used in the Kirchmeier paper is briefly described in the following paper. The assay involves counting PFU after incubation of virus with HCMV gB antiserum. The assay is described in the last paragraph of the paper. It does not mention applying this test to this clone or other monoclonal antibodies but it seems like an easy approach.

    EMBO J. 1986 Nov; 5(11): 3057–3063.

    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1167261/?page=1

    The other HCMV antibody you have, ab6499, has not, to our knowledge, been used in a neutralization assay.

    Read More

    Answer

    This antibody was derived from ascites fluid.

    Read More

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