Anti-Cytomegalovirus IE1 and IE2 antibody [CH160] - BSA and Azide free (ab53495)
Key features and details
- Mouse monoclonal [CH160] to Cytomegalovirus IE1 and IE2 - BSA and Azide free
- Suitable for: WB, ICC/IF
- Reacts with: Human cytomegalovirus
- Isotype: IgG1
Overview
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Product name
Anti-Cytomegalovirus IE1 and IE2 antibody [CH160] - BSA and Azide free -
Description
Mouse monoclonal [CH160] to Cytomegalovirus IE1 and IE2 - BSA and Azide free -
Host species
Mouse -
Specificity
ab53495 reacts with cytomegalovirus IE1 and IE2. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human cytomegalovirus -
Immunogen
Tissue, cells or virus corresponding to Cytomegalovirus IE1 and IE2. HCMV AD169 IC Extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
CH160 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 10 µg/ml.
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|
ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 10 µg/ml. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Relevance
The IE1/2 transcriptional control region of human cytomegalovirus drives the expression of the HCMV major immediate-early genes (UL123-122), which encode proteins necessary for initiation of the virus replicative cycle. Nucleotide sequence polymorphism in this region of the viral genome could account for variations in the replication of human cytomegalovirus strains. -
Database links
- Entrez Gene: 1487821 Human cytomegalovirus
- Entrez Gene: 1487822 Human cytomegalovirus
- SwissProt: P13202 Human cytomegalovirus
- SwissProt: P19893 Human cytomegalovirus
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Alternative names
- 45 kDa immediate-early protein 2 antibody
- 55 kDa immediate-early protein 2 antibody
- HCMV antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (17)
ab53495 has been referenced in 17 publications.
- Zhang X et al. Human Cytomegalovirus-IE2 Affects Embryonic Liver Development and Survival in Transgenic Mouse. Cell Mol Gastroenterol Hepatol 14:494-511 (2022). PubMed: 35569816
- Bergamelli M et al. Human Cytomegalovirus Modifies Placental Small Extracellular Vesicle Composition to Enhance Infection of Fetal Neural Cells In Vitro. Viruses 14:N/A (2022). PubMed: 36146834
- Wang H et al. Cytomegalovirus-specific neutralizing antibodies effectively prevent uncontrolled infection after allogeneic hematopoietic stem cell transplantation. iScience 25:105065 (2022). PubMed: 36147955
- Vezzani G et al. The Human Cytomegalovirus UL116 Glycoprotein Is a Chaperone to Control gH-Based Complexes Levels on Virions. Front Microbiol 12:630121 (2021). PubMed: 33889136
- Branch KM et al. Productive Infection of Human Breast Cancer Cell Lines with Human Cytomegalovirus (HCMV). Pathogens 10:N/A (2021). PubMed: 34070980