Anti-Cytomegalovirus pp28 antibody [5C3] (ab6502)
Key features and details
- Mouse monoclonal [5C3] to Cytomegalovirus pp28
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Human cytomegalovirus
- Isotype: IgG2a
Overview
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Product name
Anti-Cytomegalovirus pp28 antibody [5C3] -
Description
Mouse monoclonal [5C3] to Cytomegalovirus pp28 -
Host species
Mouse -
Specificity
Cytomegalovirus pp28 tegument protein. Slight cross reactivity with HSV1.
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Tested applications
Suitable for: WB, ELISA, ICC/IFmore details -
Species reactivity
Reacts with: Human cytomegalovirus -
Immunogen
Tissue, cells or virus corresponding to Cytomegalovirus pp28.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
> 95% IgG by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
5C3 -
Myeloma
NS1/1-Ag4-1 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6502 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/16000. Detects a band of approximately . kDa (predicted molecular weight: 28 kDa).
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ELISA |
1/102400.
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ICC/IF |
Use at an assay dependent concentration. PubMed: 20679731
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Notes |
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WB
1/16000. Detects a band of approximately . kDa (predicted molecular weight: 28 kDa). |
ELISA
1/102400. |
ICC/IF
Use at an assay dependent concentration. PubMed: 20679731 |
Target
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Relevance
Cytomegalovirus (CMV), is a genus of Herpes viruses; in humans the species is known as Human herpesvirus 5 (HHV-5). It belongs to the Betaherpesvirinae subfamily of Herpesviridae. The name means "very big cell virus". -
Database links
- Entrez Gene: 1487761 Human cytomegalovirus
- SwissProt: P13200 Human cytomegalovirus
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Alternative names
- 28 kDa structural phosphoprotein antibody
- CMV pp28 tegument protein antibody
- CMV tegument protein UL99 antibody
see all
Images
Datasheets and documents
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Datasheet download
References (12)
ab6502 has been referenced in 12 publications.
- Perera MR & Sinclair JH The Human Cytomegalovirus β2.7 Long Non-Coding RNA Prevents Induction of Reactive Oxygen Species to Maintain Viral Gene Silencing during Latency. Int J Mol Sci 23:N/A (2022). PubMed: 36232315
- Ghassabian H et al. Divide et impera: An In Silico Screening Targeting HCMV ppUL44 Processivity Factor Homodimerization Identifies Small Molecules Inhibiting Viral Replication. Viruses 13:N/A (2021). PubMed: 34065234
- Sloan E et al. MORC3, a Component of PML Nuclear Bodies, Has a Role in Restricting Herpes Simplex Virus 1 and Human Cytomegalovirus. J Virol 90:8621-33 (2016). PubMed: 27440897
- Lu Y et al. Stimulation of the Replication of ICP0-Null Mutant Herpes Simplex Virus 1 and pp71-Deficient Human Cytomegalovirus by Epstein-Barr Virus Tegument Protein BNRF1. J Virol 90:9664-9673 (2016). WB . PubMed: 27535048
- Lu Y & Everett RD Analysis of the functional interchange between the IE1 and pp71 proteins of human cytomegalovirus and ICP0 of herpes simplex virus 1. J Virol 89:3062-75 (2015). PubMed: 25552717