Anti-Cytomegalovirus pp28 antibody [5C3] (ab6502)
Key features and details
- Mouse monoclonal [5C3] to Cytomegalovirus pp28
- Suitable for: WB, ELISA
- Isotype: IgG2a
Overview
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Product name
Anti-Cytomegalovirus pp28 antibody [5C3] -
Description
Mouse monoclonal [5C3] to Cytomegalovirus pp28 -
Host species
Mouse -
Specificity
Cytomegalovirus pp28 tegument protein. Slight cross reactivity with HSV1.
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Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Other species -
Immunogen
Cytomegalovirus
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
> 95% IgG by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
5C3 -
Myeloma
NS1/1-Ag4-1 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6502 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/16000. Detects a band of approximately . kDa (predicted molecular weight: 28 kDa).
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ELISA |
1/100000.
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Notes |
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WB
1/16000. Detects a band of approximately . kDa (predicted molecular weight: 28 kDa). |
ELISA
1/100000. |
Target
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Relevance
Cytomegalovirus (CMV), is a genus of Herpes viruses; in humans the species is known as Human herpesvirus 5 (HHV-5). It belongs to the Betaherpesvirinae subfamily of Herpesviridae. The name means "very big cell virus". -
Database links
- SwissProt: P52368 Other species
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Alternative names
- 28 kDa structural phosphoprotein antibody
- CMV pp28 tegument protein antibody
- CMV tegument protein UL99 antibody
see all
Images
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Please see protocols tab for procedure. Antigen is CMV infected cell extract coated at a dilution of 1/100.
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See protocols tab for procedure. Antigen is CMV infected cell extract at 10 µg/cm.
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab6502 has been referenced in 10 publications.
- Sloan E et al. MORC3, a Component of PML Nuclear Bodies, Has a Role in Restricting Herpes Simplex Virus 1 and Human Cytomegalovirus. J Virol 90:8621-33 (2016). PubMed: 27440897
- Lu Y et al. Stimulation of the Replication of ICP0-Null Mutant Herpes Simplex Virus 1 and pp71-Deficient Human Cytomegalovirus by Epstein-Barr Virus Tegument Protein BNRF1. J Virol 90:9664-9673 (2016). WB . PubMed: 27535048
- Lu Y & Everett RD Analysis of the functional interchange between the IE1 and pp71 proteins of human cytomegalovirus and ICP0 of herpes simplex virus 1. J Virol 89:3062-75 (2015). PubMed: 25552717
- McKinney C et al. A new role for the cellular PABP repressor Paip2 as an innate restriction factor capable of limiting productive cytomegalovirus replication. Genes Dev 27:1809-20 (2013). PubMed: 23964095
- Shikhagaie M et al. The human cytomegalovirus-specific UL1 gene encodes a late-phase glycoprotein incorporated in the virion envelope. J Virol 86:4091-101 (2012). ICC/IF ; Human . PubMed: 22345456
- Engel P et al. Human cytomegalovirus UL7, a homologue of the SLAM-family receptor CD229, impairs cytokine production. Immunol Cell Biol 89:753-66 (2011). PubMed: 21670740
- Stanton RJ et al. Reconstruction of the complete human cytomegalovirus genome in a BAC reveals RL13 to be a potent inhibitor of replication. J Clin Invest 120:3191-208 (2010). ICC/IF . PubMed: 20679731
- Buchkovich NJ et al. The endoplasmic reticulum chaperone BiP/GRP78 is important in the structure and function of the human cytomegalovirus assembly compartment. J Virol 83:11421-8 (2009). PubMed: 19741001
- Fukui Y et al. Establishment of a cell-based assay for screening of compounds inhibiting very early events in the cytomegalovirus replication cycle and characterization of a compound identified using the assay. Antimicrob Agents Chemother 52:2420-7 (2008). PubMed: 18458124
- Adair R et al. Human cytomegalovirus temporally regulated gene expression in differentiated, immortalized retinal pigment epithelial cells. J Clin Virol 35:478-84 (2006). PubMed: 16388985