Anti-Cytomegalovirus pp28 antibody [5C3] (ab6502)

Submit a review Q&A (2)References (10)
ELISA - Anti-Cytomegalovirus pp28 antibody [5C3] (ab6502)
  • Western blot - Anti-Cytomegalovirus pp28 antibody [5C3] (ab6502)

Key features and details

  • Mouse monoclonal [5C3] to Cytomegalovirus pp28
  • Suitable for: WB, ELISA
  • Isotype: IgG2a

Overview

  • Product name

    Anti-Cytomegalovirus pp28 antibody [5C3]

  • Description

    Mouse monoclonal [5C3] to Cytomegalovirus pp28

  • Host species

    Mouse

  • Specificity

    Cytomegalovirus pp28 tegument protein. Slight cross reactivity with HSV1.

  • Tested applications

    Suitable for: WB, ELISAmore details

  • Species reactivity

    Reacts with: Other species

  • Immunogen

    Cytomegalovirus

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    pH: 7.40
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    > 95% IgG by SDS-PAGE.

  • Clonality

    Monoclonal

  • Clone number

    5C3

  • Myeloma

    NS1/1-Ag4-1

  • Isotype

    IgG2a

  • Light chain type

    kappa

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/16000. Detects a band of approximately . kDa (predicted molecular weight: 28 kDa).
ELISA
1/100000.
Notes
WB
1/16000. Detects a band of approximately . kDa (predicted molecular weight: 28 kDa).
ELISA
1/100000.

Target

  • Relevance

    Cytomegalovirus (CMV), is a genus of Herpes viruses; in humans the species is known as Human herpesvirus 5 (HHV-5). It belongs to the Betaherpesvirinae subfamily of Herpesviridae. The name means "very big cell virus".

  • Database links

    • Alternative names

      • 28 kDa structural phosphoprotein antibody
      • CMV pp28 tegument protein antibody
      • CMV tegument protein UL99 antibody
      • Cytomegalovirus pp28 tegument protein antibody
      • Cytomegalovirus tegument protein UL99 antibody
      • HCMV antibody
      • HHV 5 antibody
      • Human Herpesvirus 5 antibody
      • Tegument protein UL99 antibody
      • UL 99 antibody
      • UL99 antibody
      see all

    Images

    • ELISA - Anti-Cytomegalovirus pp28 antibody [5C3] (ab6502)
      ELISA - Anti-Cytomegalovirus pp28 antibody [5C3] (ab6502)

      Please see protocols tab for procedure. Antigen is CMV infected cell extract coated at a dilution of 1/100.

    • Western blot - Anti-Cytomegalovirus pp28 antibody [5C3] (ab6502)
      Western blot - Anti-Cytomegalovirus pp28 antibody [5C3] (ab6502)

      See protocols tab for procedure. Antigen is CMV infected cell extract at 10 µg/cm.

    References (10)

    Publishing research using ab6502? Please let us know so that we can cite the reference in this datasheet.

    ab6502 has been referenced in 10 publications.

    • Sloan E  et al. MORC3, a Component of PML Nuclear Bodies, Has a Role in Restricting Herpes Simplex Virus 1 and Human Cytomegalovirus. J Virol 90:8621-33 (2016). PubMed: 27440897
    • Lu Y  et al. Stimulation of the Replication of ICP0-Null Mutant Herpes Simplex Virus 1 and pp71-Deficient Human Cytomegalovirus by Epstein-Barr Virus Tegument Protein BNRF1. J Virol 90:9664-9673 (2016). WB . PubMed: 27535048
    • Lu Y & Everett RD Analysis of the functional interchange between the IE1 and pp71 proteins of human cytomegalovirus and ICP0 of herpes simplex virus 1. J Virol 89:3062-75 (2015). PubMed: 25552717
    • McKinney C  et al. A new role for the cellular PABP repressor Paip2 as an innate restriction factor capable of limiting productive cytomegalovirus replication. Genes Dev 27:1809-20 (2013). PubMed: 23964095
    • Shikhagaie M  et al. The human cytomegalovirus-specific UL1 gene encodes a late-phase glycoprotein incorporated in the virion envelope. J Virol 86:4091-101 (2012). ICC/IF ; Human . PubMed: 22345456
    • Engel P  et al. Human cytomegalovirus UL7, a homologue of the SLAM-family receptor CD229, impairs cytokine production. Immunol Cell Biol 89:753-66 (2011). PubMed: 21670740
    • Stanton RJ  et al. Reconstruction of the complete human cytomegalovirus genome in a BAC reveals RL13 to be a potent inhibitor of replication. J Clin Invest 120:3191-208 (2010). ICC/IF . PubMed: 20679731
    • Buchkovich NJ  et al. The endoplasmic reticulum chaperone BiP/GRP78 is important in the structure and function of the human cytomegalovirus assembly compartment. J Virol 83:11421-8 (2009). PubMed: 19741001
    • Fukui Y  et al. Establishment of a cell-based assay for screening of compounds inhibiting very early events in the cytomegalovirus replication cycle and characterization of a compound identified using the assay. Antimicrob Agents Chemother 52:2420-7 (2008). PubMed: 18458124
    • Adair R  et al. Human cytomegalovirus temporally regulated gene expression in differentiated, immortalized retinal pigment epithelial cells. J Clin Virol 35:478-84 (2006). PubMed: 16388985

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    thaks for your answer,i'd like to know too about antibody ab6502, does it recognizes pp28 protein form HCMV or from MCMV? dou you have it in stock? waht is the price? thaks again

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    Answer

    Thank you for your enquiry. I can confirm that this antibody (ab6502) has been successfully tested for detection of human CMV pp28. It would also be predicted to detect murine CMV pp28, although this has not been tested and cannot be guaranteed. I hope this information is helpful. Should you have any further questions, please do not hesitate to contact us again.

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    Question

    I have now tested the anti-p28 antibody we recently obtained from abcam (ab6502) using the dilution and fixation conditions you suggest. The antibody really does not detect what it is meant to. On infected cells the anti-p28 antibody is intense but not specific (it does show more intense staining than an isotype matched control antibody but, as I said, it appears totally non-specific and stains all of the cell very intensely). Perhaps worse, is the extremely intense punctate nuclear fluorescence it shows on un-infected cells (which is not shown with an isotype matched control). Clearly, this antibody is not specific for HCMV infected cells and does not detect pp28 expression as detailed in your data sheet. I am happy to try another Lot number if you think there may be problems with the batch we have received. Alternatively, if you can put us in contact with anyone in the UK or Europe who has purchased this antibody, we would be happy to contact them regarding our problems. Failing that, we would appreciate a refund on the purchase cost of the antibody and be happy to send you back the antibody which we have.

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    Answer

    Thank you for your enquiry. We are very sorry that you are dissatisfied with this particular antibody. We take your complaint very seriously and certainly we can offer you either a new vial - free of charge or a refund. Please do let us know which you would prefer. However, we would like to investigate this matter further and would highly appreciate if you could provide us a detailed protocol. This background information would help us to decide if the antibody is really specific for detecting Cytomegalovirus pp28. If not, then we will unpublish the product and withdraw it from our catalogue. 1. Please describe the problem (high background, no staining etc). 2. On what material are you testing the antibody in IHC? • Species? • Cell line? • Tissue? 3. How did you fix the samples? • Ethanol, methanol • Acetone • Paraformaldehyde • Other 4. Did you apply antigen retrieval step? • Enzymatic method • Heat mediated technique • Other 5. How did you block the unspecific binding sites? 6. Primary antibody • Specification (in which species was it raised against)? • At what dilution(s) have you tested this antibody? • Incubation time, wash steps (multiple short washes are more effective than fewer longer wash steps)? 7. Secondary antibody • What secondary antibody are you using? • Specification (in which species was it raised against)? • At what dilution(s) have you tested this antibody? • Incubation, wash steps? • Do you know whether the problems you are experiencing come from the secondary? • What detection method are you using? 8. Background staining • Please provide an image of your staining 9. Which detection system did you use? 10. Did you apply positive and negative controls along with the samples? Please specify. 11. Optimization attempts • How many times have you tried the IHC? • Do you obtain the same results every time? • What steps have you altered? Thank you for your understanding and your co-operation. We are looking forward to hearing from you soon.

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