Anti-Cytomegalovirus pp28 antibody [5C3] (ab6502)

Submit a review Q&A (2)References (10)

Overview

  • Product name
    Anti-Cytomegalovirus pp28 antibody [5C3]
  • Description
    Mouse monoclonal [5C3] to Cytomegalovirus pp28
  • Host species
    Mouse
  • Specificity
    Cytomegalovirus pp28 tegument protein. Slight cross reactivity with HSV1 (please see image 116).
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Immunogen

    Cytomegalovirus

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    > 95% IgG by SDS-PAGE.
  • Clonality
    Monoclonal
  • Clone number
    5C3
  • Myeloma
    NS1/1-Ag4-1
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/16000. Detects a band of approximately . kDa (predicted molecular weight: 28 kDa).
ELISA 1/100000.
ICC/IF Use at an assay dependent dilution. PubMed: 20679731

Target

  • Relevance
    Cytomegalovirus (CMV), is a genus of Herpes viruses; in humans the species is known as Human herpesvirus 5 (HHV-5). It belongs to the Betaherpesvirinae subfamily of Herpesviridae. The name means "very big cell virus".
  • Alternative names
    • 28 kDa structural phosphoprotein antibody
    • CMV pp28 tegument protein antibody
    • CMV tegument protein UL99 antibody
    • Cytomegalovirus pp28 tegument protein antibody
    • Cytomegalovirus tegument protein UL99 antibody
    • HCMV antibody
    • HHV 5 antibody
    • Human Herpesvirus 5 antibody
    • Tegument protein UL99 antibody
    • UL 99 antibody
    • UL99 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytomegalovirus pp28 antibody [5C3] (ab6502)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytomegalovirus pp28 antibody [5C3] (ab6502)

    ab6502 at a 1/200 dilution staining CMV pp28 in CMV (AD169) infected human foreskin fibroblasts. Please see protocols tab for procedure.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytomegalovirus pp28 antibody [5C3] (ab6502)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytomegalovirus pp28 antibody [5C3] (ab6502)

     

  • ELISA - Anti-Cytomegalovirus pp28 antibody [5C3] (ab6502)
    ELISA - Anti-Cytomegalovirus pp28 antibody [5C3] (ab6502)

    Please see protocols tab for procedure. Antigen is CMV infected cell extract coated at a dilution of 1/100.

  • Western blot - Anti-Cytomegalovirus pp28 antibody [5C3] (ab6502)
    Western blot - Anti-Cytomegalovirus pp28 antibody [5C3] (ab6502)

    See protocols tab for procedure. Antigen is CMV infected cell extract at 10 µg/cm.

    See protocols tab for procedure. Antigen is CMV infected cell extract at 10 µg/cm.

References

This product has been referenced in:
  • Sloan E  et al. MORC3, a Component of PML Nuclear Bodies, Has a Role in Restricting Herpes Simplex Virus 1 and Human Cytomegalovirus. J Virol 90:8621-33 (2016). Read more (PubMed: 27440897) »
  • Lu Y  et al. Stimulation of the Replication of ICP0-Null Mutant Herpes Simplex Virus 1 and pp71-Deficient Human Cytomegalovirus by Epstein-Barr Virus Tegument Protein BNRF1. J Virol 90:9664-9673 (2016). WB . Read more (PubMed: 27535048) »
See all 10 Publications for this product

Publishing research using ab6502? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

thaks for your answer,i'd like to know too about antibody ab6502, does it recognizes pp28 protein form HCMV or from MCMV? dou you have it in stock? waht is the price? thaks again

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Answer

Thank you for your enquiry. I can confirm that this antibody (ab6502) has been successfully tested for detection of human CMV pp28. It would also be predicted to detect murine CMV pp28, although this has not been tested and cannot be guaranteed. I hope this information is helpful. Should you have any further questions, please do not hesitate to contact us again.

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Question

I have now tested the anti-p28 antibody we recently obtained from abcam (ab6502) using the dilution and fixation conditions you suggest. The antibody really does not detect what it is meant to. On infected cells the anti-p28 antibody is intense but not specific (it does show more intense staining than an isotype matched control antibody but, as I said, it appears totally non-specific and stains all of the cell very intensely). Perhaps worse, is the extremely intense punctate nuclear fluorescence it shows on un-infected cells (which is not shown with an isotype matched control). Clearly, this antibody is not specific for HCMV infected cells and does not detect pp28 expression as detailed in your data sheet. I am happy to try another Lot number if you think there may be problems with the batch we have received. Alternatively, if you can put us in contact with anyone in the UK or Europe who has purchased this antibody, we would be happy to contact them regarding our problems. Failing that, we would appreciate a refund on the purchase cost of the antibody and be happy to send you back the antibody which we have.

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Answer

Thank you for your enquiry. We are very sorry that you are dissatisfied with this particular antibody. We take your complaint very seriously and certainly we can offer you either a new vial - free of charge or a refund. Please do let us know which you would prefer. However, we would like to investigate this matter further and would highly appreciate if you could provide us a detailed protocol. This background information would help us to decide if the antibody is really specific for detecting Cytomegalovirus pp28. If not, then we will unpublish the product and withdraw it from our catalogue. 1. Please describe the problem (high background, no staining etc). 2. On what material are you testing the antibody in IHC? • Species? • Cell line? • Tissue? 3. How did you fix the samples? • Ethanol, methanol • Acetone • Paraformaldehyde • Other 4. Did you apply antigen retrieval step? • Enzymatic method • Heat mediated technique • Other 5. How did you block the unspecific binding sites? 6. Primary antibody • Specification (in which species was it raised against)? • At what dilution(s) have you tested this antibody? • Incubation time, wash steps (multiple short washes are more effective than fewer longer wash steps)? 7. Secondary antibody • What secondary antibody are you using? • Specification (in which species was it raised against)? • At what dilution(s) have you tested this antibody? • Incubation, wash steps? • Do you know whether the problems you are experiencing come from the secondary? • What detection method are you using? 8. Background staining • Please provide an image of your staining 9. Which detection system did you use? 10. Did you apply positive and negative controls along with the samples? Please specify. 11. Optimization attempts • How many times have you tried the IHC? • Do you obtain the same results every time? • What steps have you altered? Thank you for your understanding and your co-operation. We are looking forward to hearing from you soon.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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