Anti-Cytomegalovirus pp28 antibody [5C3] (ab6502)

Thank you for your enquiry. I can confirm that this antibody (ab6502) has been successfully tested for detection of human CMV pp28. It would also be predicted to detect murine CMV pp28, although this has not been tested and cannot be guaranteed. I hope this information is helpful. Should you have any further questions, please do not hesitate to contact us again.

Thank you for your enquiry. We are very sorry that you are dissatisfied with this particular antibody. We take your complaint very seriously and certainly we can offer you either a new vial - free of charge or a refund. Please do let us know which you would prefer. However, we would like to investigate this matter further and would highly appreciate if you could provide us a detailed protocol. This background information would help us to decide if the antibody is really specific for detecting Cytomegalovirus pp28. If not, then we will unpublish the product and withdraw it from our catalogue. 1. Please describe the problem (high background, no staining etc). 2. On what material are you testing the antibody in IHC? • Species? • Cell line? • Tissue? 3. How did you fix the samples? • Ethanol, methanol • Acetone • Paraformaldehyde • Other 4. Did you apply antigen retrieval step? • Enzymatic method • Heat mediated technique • Other 5. How did you block the unspecific binding sites? 6. Primary antibody • Specification (in which species was it raised against)? • At what dilution(s) have you tested this antibody? • Incubation time, wash steps (multiple short washes are more effective than fewer longer wash steps)? 7. Secondary antibody • What secondary antibody are you using? • Specification (in which species was it raised against)? • At what dilution(s) have you tested this antibody? • Incubation, wash steps? • Do you know whether the problems you are experiencing come from the secondary? • What detection method are you using? 8. Background staining • Please provide an image of your staining 9. Which detection system did you use? 10. Did you apply positive and negative controls along with the samples? Please specify. 11. Optimization attempts • How many times have you tried the IHC? • Do you obtain the same results every time? • What steps have you altered? Thank you for your understanding and your co-operation. We are looking forward to hearing from you soon.