Anti-Cytomegalovirus pp65 antibody [1-L-11] (ab31624)

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Overview

  • Product name

    Anti-Cytomegalovirus pp65 antibody [1-L-11]
    See all Cytomegalovirus pp65 primary antibodies

  • Description

    Mouse monoclonal [1-L-11] to Cytomegalovirus pp65

  • Host species

    Mouse

  • Specificity

    Recognizes human pp65 immediate early nuclear antigen. The clone number has been updated from (8.V.035) to (1-L-11) both clone numbers name the same antibody clone.

  • Tested applications

    Suitable for: WB, ELISA, ICC/IFmore details

  • Species reactivity

    Reacts with: Other species

  • Immunogen

    Infected HCMV cells (Human)

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    1-L-11

  • Isotype

    IgG1

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab31624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500.
ELISA 1/5000.
ICC/IF Use at an assay dependent dilution.

Target

  • Relevance

    Cytomegalovirus is a member of the herpes virus group, which includes herpes simplex virus types 1 and 2, varicella zoster virus (which causes chicken pox), and Epstein Barr virus (which causes infectious mononucleosis). These viruses share a characteristic ability to remain dormant within the body over a long period. CMV viral genes are co-ordinately expressed in groups at various times after infection. Early viral proteins are expressed in the nucleus of infected cells within 3 to 24 hours of infection prior to the commencement of viral DNA replication. This is followed by expression of the early intermediate genes, which encode enzymes required for viral DNA replication. After 48 to 72 hours, a number of late viral antigens may be demonstrated in the nuclei and cytoplasm of infected cells. pp65 is a 65kD phosphorylated glycoprotein and is the most abundant of the late antigens.

  • Alternative names

    • 65 kDa matrix phosphoprotein antibody
    • 65 kDa phosphoprotein antibody
    • Tegument protein pp65 antibody
    • Tegument protein UL83 antibody

References

This product has been referenced in:

  • Shimizu D  et al. Infection of endotheliotropic human cytomegalovirus of trabecular meshwork cells. Jpn J Ophthalmol 62:667-676 (2018). Read more (PubMed: 30191418) »
See 1 Publication for this product

Publishing research using ab31624? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

Hi, We are interested in antibodies against human CMV pp65. We don't need the antibody for the whole pp65 protein, rather we need the AB for a certain epitope. please send us your info. Thanks.

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Answer

Thank you for your inquiry. As you may have seen already, we have a number of HCMV pp65 antibodies available in our catalog (such as ab6503, ab8250, ab31624, ab49214, ab53489). These were raised against the pp65 protein, however, unfortunately the exact epitope recognised by each antibody has not been mapped, as epitope mapping is not routinely performed.  I am sorry that I cannot be more helpful on this occasion. Please do not hesitate to contact us if you have any further questions.  

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