Anti-Cytomegalovirus pp65 antibody [1-L-11] (ab31624)
Key features and details
- Mouse monoclonal [1-L-11] to Cytomegalovirus pp65
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Human cytomegalovirus
- Isotype: IgG1
Overview
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Product name
Anti-Cytomegalovirus pp65 antibody [1-L-11]
See all Cytomegalovirus pp65 primary antibodies -
Description
Mouse monoclonal [1-L-11] to Cytomegalovirus pp65 -
Host species
Mouse -
Specificity
Recognizes human pp65 immediate early nuclear antigen. The clone number has been updated from (8.V.035) to (1-L-11) both clone numbers name the same antibody clone. -
Tested applications
Suitable for: WB, ELISA, ICC/IFmore details -
Species reactivity
Reacts with: Human cytomegalovirus -
Immunogen
Tissue, cells or virus corresponding to Human Cytomegalovirus pp65.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
1-L-11 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab31624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500.
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| ELISA |
1/5000.
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| ICC/IF |
Use at an assay dependent dilution.
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| Notes |
|---|
|
WB
1/500. |
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ELISA
1/5000. |
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ICC/IF
Use at an assay dependent dilution. |
Target
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Relevance
Cytomegalovirus is a member of the herpes virus group, which includes herpes simplex virus types 1 and 2, varicella zoster virus (which causes chicken pox), and Epstein Barr virus (which causes infectious mononucleosis). These viruses share a characteristic ability to remain dormant within the body over a long period. CMV viral genes are co-ordinately expressed in groups at various times after infection. Early viral proteins are expressed in the nucleus of infected cells within 3 to 24 hours of infection prior to the commencement of viral DNA replication. This is followed by expression of the early intermediate genes, which encode enzymes required for viral DNA replication. After 48 to 72 hours, a number of late viral antigens may be demonstrated in the nuclei and cytoplasm of infected cells. pp65 is a 65kD phosphorylated glycoprotein and is the most abundant of the late antigens. -
Database links
- Entrez Gene: 1487754 Human cytomegalovirus
- SwissProt: P06725 Human cytomegalovirus
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Alternative names
- 65 kDa matrix phosphoprotein antibody
- 65 kDa phosphoprotein antibody
- Tegument protein pp65 antibody
- Tegument protein UL83 antibody
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab31624 has been referenced in 2 publications.
- Ma YS et al. MicroRNA-499 serves as a sensitizer for lung cancer cells to radiotherapy by inhibition of CK2a-mediated phosphorylation of p65. Mol Ther Oncolytics 21:171-182 (2021). PubMed: 33997273
- Shimizu D et al. Infection of endotheliotropic human cytomegalovirus of trabecular meshwork cells. Jpn J Ophthalmol 62:667-676 (2018). PubMed: 30191418