Overview

  • Product name

    Anti-Cytomegalovirus pp65 antibody [3A12] - Azide free
    See all Cytomegalovirus pp65 primary antibodies
  • Description

    Mouse monoclonal [3A12] to Cytomegalovirus pp65 - Azide free
  • Host species

    Mouse
  • Specificity

    Cytomegalovirus pp65 (Lower Matrix Protein).
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Cytomegalovirus

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.4
    Constituent: 100% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    3A12
  • Myeloma

    NS1/1-Ag4-1
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/16000.
ICC/IF 1/3200.

Target

  • Relevance

    Cytomegalovirus is a member of the herpes virus group, which includes herpes simplex virus types 1 and 2, varicella zoster virus (which causes chicken pox), and Epstein Barr virus (which causes infectious mononucleosis). These viruses share a characteristic ability to remain dormant within the body over a long period. CMV viral genes are co-ordinately expressed in groups at various times after infection. Early viral proteins are expressed in the nucleus of infected cells within 3 to 24 hours of infection prior to the commencement of viral DNA replication. This is followed by expression of the early intermediate genes, which encode enzymes required for viral DNA replication. After 48 to 72 hours, a number of late viral antigens may be demonstrated in the nuclei and cytoplasm of infected cells. pp65 is a 65kD phosphorylated glycoprotein and is the most abundant of the late antigens.
  • Alternative names

    • 65 kDa matrix phosphoprotein antibody
    • 65 kDa phosphoprotein antibody
    • Tegument protein pp65 antibody
    • Tegument protein UL83 antibody

References

This product has been referenced in:

  • Liu X  et al. Human cytomegalovirus evades antibody-mediated immunity through endoplasmic reticulum-associated degradation of the FcRn receptor. Nat Commun 10:3020 (2019). Read more (PubMed: 31289263) »
  • Chen X & Zhu B Steroid Receptor Coactivator-Interacting Protein (SIP) Suppresses Myocardial Injury Caused by Acute Pancreatitis. Med Sci Monit 24:3204-3211 (2018). Read more (PubMed: 29765014) »
See all 9 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your inquiry. As you may have seen already, we have a number of HCMV pp65 antibodies available in our catalog (such as ab6503, ab8250, ab31624, ab49214, ab53489). These were raised against the pp65 protein, however, unfortunately the exact epitope recognised by each antibody has not been mapped, as epitope mapping is not routinely performed.  I am sorry that I cannot be more helpful on this occasion. Please do not hesitate to contact us if you have any further questions.  

Read More

Answer

I put your question forward to the originator but they said that the epitope has not been characterised so they do not have the information. I am sorry we cannot be of help to you.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up