• Product name

    Anti-Cytomegalovirus pp65 antibody [CH12]
    See all Cytomegalovirus pp65 primary antibodies
  • Description

    Mouse monoclonal [CH12] to Cytomegalovirus pp65
  • Host species

  • Specificity

    ab53489 is reactive with pp65 (UL83) of Cytomegalovirus.
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    HCMV AD169 IC Extract.


  • Form

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: None
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

  • Clone number

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab53489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 10 µg/ml.


  • Relevance

    Cytomegalovirus is a member of the herpes virus group, which includes herpes simplex virus types 1 and 2, varicella zoster virus (which causes chicken pox), and Epstein Barr virus (which causes infectious mononucleosis). These viruses share a characteristic ability to remain dormant within the body over a long period. CMV viral genes are co-ordinately expressed in groups at various times after infection. Early viral proteins are expressed in the nucleus of infected cells within 3 to 24 hours of infection prior to the commencement of viral DNA replication. This is followed by expression of the early intermediate genes, which encode enzymes required for viral DNA replication. After 48 to 72 hours, a number of late viral antigens may be demonstrated in the nuclei and cytoplasm of infected cells. pp65 is a 65kD phosphorylated glycoprotein and is the most abundant of the late antigens.
  • Alternative names

    • 65 kDa matrix phosphoprotein antibody
    • 65 kDa phosphoprotein antibody
    • Tegument protein pp65 antibody
    • Tegument protein UL83 antibody


This product has been referenced in:

  • Kiener R  et al. Vaccine vectors based on Adenovirus 19a/64 exhibit broad cellular tropism and potently restimulate HCMV-specific T cell responses ex vivo. Sci Rep 8:1474 (2018). Read more (PubMed: 29367743) »
  • Kiener R  et al. Efficient Delivery of Human Cytomegalovirus T Cell Antigens by Attenuated Sendai Virus Vectors. J Virol 92:N/A (2018). Read more (PubMed: 29769344) »
See all 3 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A


Thank you for your reply.

The polyclonal isotope control will work with the monoclonal antibody. You should have no issues using this combination.

Please let me know if there is anything else I can help you with.

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Thank you for your reply.

The secondary antibody ab130782 would be a good choice to use with ab53495, and the isotope that I mentioned previously would also work well.

If I can help you select any other reagents, or if there is anything else I can help you with, please let me know.

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Thank you for contacting Abcam.

For ab53489, I would recommend the following mouse IgG Isotype control:


If there is anything else I can help you with, please let me know.

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Thank you for your inquiry. As you may have seen already, we have a number of HCMV pp65 antibodies available in our catalog (such as ab6503, ab8250, ab31624, ab49214, ab53489). These were raised against the pp65 protein, however, unfortunately the exact epitope recognised by each antibody has not been mapped, as epitope mapping is not routinely performed.  I am sorry that I cannot be more helpful on this occasion. Please do not hesitate to contact us if you have any further questions.  

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