Key features and details
- Mouse monoclonal [CH12] to Cytomegalovirus pp65
- Suitable for: ICC/IF, WB
- Isotype: IgG
Product nameAnti-Cytomegalovirus pp65 antibody [CH12]
See all Cytomegalovirus pp65 primary antibodies
DescriptionMouse monoclonal [CH12] to Cytomegalovirus pp65
Specificityab53489 is reactive with pp65 (UL83) of Cytomegalovirus.
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Other species
HCMV AD169 IC Extract.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab53489 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 µg/ml.|
|WB||Use a concentration of 10 µg/ml.|
RelevanceCytomegalovirus is a member of the herpes virus group, which includes herpes simplex virus types 1 and 2, varicella zoster virus (which causes chicken pox), and Epstein Barr virus (which causes infectious mononucleosis). These viruses share a characteristic ability to remain dormant within the body over a long period. CMV viral genes are co-ordinately expressed in groups at various times after infection. Early viral proteins are expressed in the nucleus of infected cells within 3 to 24 hours of infection prior to the commencement of viral DNA replication. This is followed by expression of the early intermediate genes, which encode enzymes required for viral DNA replication. After 48 to 72 hours, a number of late viral antigens may be demonstrated in the nuclei and cytoplasm of infected cells. pp65 is a 65kD phosphorylated glycoprotein and is the most abundant of the late antigens.
- 65 kDa matrix phosphoprotein antibody
- 65 kDa phosphoprotein antibody
- Tegument protein pp65 antibody
- Tegument protein UL83 antibody
ab53489 has been referenced in 4 publications.
- Kiener R et al. Vaccine vectors based on Adenovirus 19a/64 exhibit broad cellular tropism and potently restimulate HCMV-specific T cell responses ex vivo. Sci Rep 8:1474 (2018). PubMed: 29367743
- Kiener R et al. Efficient Delivery of Human Cytomegalovirus T Cell Antigens by Attenuated Sendai Virus Vectors. J Virol 92:N/A (2018). PubMed: 29769344
- Wu Z et al. Relationship between T-cell receptor beta chain sequences and human cytomegalovirus infection in allogeneic hematopoietic stem cell transplant recipients. Mol Med Rep 15:3898-3904 (2017). PubMed: 28440401
- Walker JD et al. Cytomegalovirus-infected human endothelial cells can stimulate allogeneic CD4+ memory T cells by releasing antigenic exosomes. J Immunol 182:1548-59 (2009). WB . PubMed: 19155503