Recombinant Anti-DLC-A antibody [EPR11240] (ab157468)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11240] to DLC-A
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-DLC-A antibody [EPR11240]
See all DLC-A primary antibodies -
Description
Rabbit monoclonal [EPR11240] to DLC-A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human DLC-A.
Database link: Q9Y6G9 -
Positive control
- K562, HeLa, fetal brain, fetal heart and Jurkat lysates; Human heart and pancreas tissues; K562 cells; Immunoprecipitation pellet from fetal brain lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11240 -
Isotype
IgG
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab157468 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 57 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
Target
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Function
Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in binding dynein to membranous organelles or chromosomes. Probably involved in the microtubule-dependent transport of pericentrin. Is required for progress throuh the spindle assembly checkpoint. The phosphorylated form appears to be involved in the selective removal of MAD1L1 and MAD1L2 but not BUB1B from kinetochores. -
Sequence similarities
Belongs to the dynein light intermediate chain family. -
Post-translational
modificationsPhosphorylated during mitosis but not in interphase. -
Cellular localization
Cytoplasm. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle pole. - Information by UniProt
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Database links
- Entrez Gene: 51143 Human
- Entrez Gene: 235661 Mouse
- Entrez Gene: 252902 Rat
- SwissProt: Q9Y6G9 Human
- SwissProt: Q8R1Q8 Mouse
- SwissProt: Q9QXU8 Rat
- Unigene: 529495 Human
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Alternative names
- Cytoplasmic dynein 1 light intermediate chain 1 antibody
- cytosolic antibody
- DC1L1_HUMAN antibody
see all
Images
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All lanes : Anti-DLC-A antibody [EPR11240] (ab157468) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Fetal brain tissue lysate
Lane 4 : Fetal heart tissue lysate
Lane 5 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 57 kDa -
Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling DLC-A with ab157468 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling DLC-A with ab157468 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent analysis of K562 cells labeling DLC-A with ab157468 at 1/100 dilution.
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Anti-DLC-A antibody [EPR11240] (ab157468) at 1/1000 dilution + immunoprecipitation pellet from fetal brain lysate
Secondary
HRP-conjugated anti-rabbit IgG, which preferentially detects the non-reduced form of rabbit IgG.
Predicted band size: 57 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab157468 has been referenced in 7 publications.
- Zhang Y et al. Dync1li1 is required for the survival of mammalian cochlear hair cells by regulating the transportation of autophagosomes. PLoS Genet 18:e1010232 (2022). PubMed: 35727824
- Cason SE et al. Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathway. J Cell Biol 220:N/A (2021). PubMed: 34014261
- Ubukawa K et al. Cdc42 regulates cell polarization and contractile actomyosin rings during terminal differentiation of human erythroblasts. Sci Rep 10:11806 (2020). PubMed: 32678227
- Villari G et al. Distinct retrograde microtubule motor sets drive early and late endosome transport. EMBO J 39:e103661 (2020). PubMed: 33215754
- Neubauer HA et al. Cytoplasmic dynein regulates the subcellular localization of sphingosine kinase 2 to elicit tumor-suppressive functions in glioblastoma. Oncogene 38:1151-1165 (2019). PubMed: 30250299
- Ma J et al. Mechanisms by Which Dendritic Cells Present Tumor Microparticle Antigens to CD8+ T Cells. Cancer Immunol Res 6:1057-1068 (2018). PubMed: 30018046
- Itoh Y et al. PDK1-Akt pathway regulates radial neuronal migration and microtubules in the developing mouse neocortex. Proc Natl Acad Sci U S A 113:E2955-64 (2016). PubMed: 27170189