Product nameAnti-Cytosolic Phospholipase A2 antibody
See all Cytosolic Phospholipase A2 primary antibodies
DescriptionRabbit polyclonal to Cytosolic Phospholipase A2
Specificityab139388 detects endogenous levels of total Cytosolic Phospholipase A2 protein.
Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human Cytosolic Phospholipase A2.
- Human uterus and tonsil tissues; HeLa cells treated with TNF alpha; HuvEc cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol
PBS is without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipoprotein metabolism
Our Abpromise guarantee covers the use of ab139388 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 85 kDa.|
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/500.|
FunctionSelectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.
Tissue specificityExpressed in various tissues such as macrophages, platelets, neutrophils, fibroblasts and lung endothelium.
Sequence similaritiesContains 1 C2 domain.
Contains 1 PLA2c domain.
DomainThe N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca(2+).
modificationsActivated by phosphorylation at both Ser-505 and Ser-727.
Cellular localizationCytoplasm. Cytoplasmic vesicle. Translocates to membrane vesicles in a calcium-dependent fashion.
- Information by UniProt
- Calcium dependent phospholipid binding protein antibody
- CPLA 2 antibody
- cPLA2 alpha antibody
All lanes : Anti-Cytosolic Phospholipase A2 antibody (ab139388) at 1/500 dilution
Lane 1 : HeLa cell lysate treated with TNF alpha at 20 ng/ml for 30 minutes
Lane 2 : HeLa cell lysate treated with TNF alpha at 20 ng/ml for 30 minutes, and also treated with the synthesized peptide
Predicted band size: 85 kDa
Immunohistochemical analysis of formalin fixed, paraffin embedded Human tonsil tissue labeled with ab139388 at 10 µg/ml.
Immunohistochemical analysis of formalin fixed, paraffin embedded Human uterus tissue labeled with ab139388 at 10 µg/ml.
Immunofluorescence analysis of HuvEc cells labeling Cytosolic Phospholipase A2 with ab139388 at a 1/100 dilution. The picture on the right is treated with the synthesized peptide.
ab139388 has not yet been referenced specifically in any publications.