Overview

  • Product name

    Anti-Cytosolic Phospholipase A2 antibody
    See all Cytosolic Phospholipase A2 primary antibodies
  • Description

    Rabbit polyclonal to Cytosolic Phospholipase A2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Synthetic peptide within Human Cytosolic Phospholipase A2 aa 699-749. The exact sequence is proprietary.
    Database link: P47712

  • Positive control

    • WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab226471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 85 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.
  • Tissue specificity

    Expressed in various tissues such as macrophages, platelets, neutrophils, fibroblasts and lung endothelium.
  • Sequence similarities

    Contains 1 C2 domain.
    Contains 1 PLA2c domain.
  • Domain

    The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca(2+).
  • Post-translational
    modifications

    Activated by phosphorylation at both Ser-505 and Ser-727.
  • Cellular localization

    Cytoplasm. Cytoplasmic vesicle. Translocates to membrane vesicles in a calcium-dependent fashion.
  • Information by UniProt
  • Database links

  • Alternative names

    • Calcium dependent phospholipid binding protein antibody
    • CPLA 2 antibody
    • cPLA2 alpha antibody
    • cPLA2 antibody
    • Cytosolic phospholipase A2 antibody
    • Cytosolic phospholipase A2 group IVA antibody
    • Lysophospholipase antibody
    • MGC126350 antibody
    • PA24A_HUMAN antibody
    • Phosphatidylcholine 2 acylhydrolase antibody
    • Phosphatidylcholine 2-acylhydrolase antibody
    • Phospholipase A2 group 4 A antibody
    • Phospholipase A2 group IVA (cytosolic calcium dependent) antibody
    • Phospholipase A2 group IVA antibody
    • PhospholipaseA2 antibody
    • PLA2G4 antibody
    • pla2g4a antibody
    see all

Images

  • All lanes : Anti-Cytosolic Phospholipase A2 antibody (ab226471) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 85 kDa


    Exposure time: 3 minutes


    Lysates prepared using NETN lysis buffer.

  • Cytosolic Phospholipase A2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226471 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226471 at 1 µg/ml.

    Lane 1: ab226471 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence with exposure time of 30 seconds.

References

ab226471 has not yet been referenced specifically in any publications.

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