Anti-Cytosolic Phospholipase A2 antibody [C1] (ab239730)
Key features and details
- Mouse monoclonal [C1] to Cytosolic Phospholipase A2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-Cytosolic Phospholipase A2 antibody [C1]
See all Cytosolic Phospholipase A2 primary antibodies -
Description
Mouse monoclonal [C1] to Cytosolic Phospholipase A2 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Orangutan -
Immunogen
Recombinant fragment corresponding to Human Cytosolic Phospholipase A2 aa 550-750. N-Terminal His-tag. Expressed in E.coli
Database link: P47712 -
Positive control
- WB: Recombinant human Cytosolic Phospholipase A2 protein; human lung lysate; PC3 and HeLa cell lysates. IHC-P: Human kidney, pancreas, glioma and stomach tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
C1 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab239730 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 85 kDa.
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IHC-P |
Use a concentration of 5 - 30 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 85 kDa. |
IHC-P
Use a concentration of 5 - 30 µg/ml. |
Target
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Function
Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response. -
Tissue specificity
Expressed in various tissues such as macrophages, platelets, neutrophils, fibroblasts and lung endothelium. -
Sequence similarities
Contains 1 C2 domain.
Contains 1 PLA2c domain. -
Domain
The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca(2+). -
Post-translational
modificationsActivated by phosphorylation at both Ser-505 and Ser-727. -
Cellular localization
Cytoplasm. Cytoplasmic vesicle. Translocates to membrane vesicles in a calcium-dependent fashion. - Information by UniProt
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Database links
- Entrez Gene: 525072 Cow
- Entrez Gene: 100033889 Horse
- Entrez Gene: 151056 Human
- Entrez Gene: 5321 Human
- Entrez Gene: 18783 Mouse
- Entrez Gene: 665270 Mouse
- Entrez Gene: 100173125 Orangutan
- Entrez Gene: 100008748 Rabbit
see all -
Alternative names
- Calcium dependent phospholipid binding protein antibody
- CPLA 2 antibody
- cPLA2 alpha antibody
see all
Images
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Paraffin-embedded human glioma tissue stained for Cytosolic Phospholipase A2 using ab239730 at 30 μg/ml in immunohistochemical analysis. DAB staining.
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All lanes : Anti-Cytosolic Phospholipase A2 antibody [C1] (ab239730) at 1 µg/ml
Lane 1 : Human lung lysate
Lane 2 : PC-3 (human prostate adenocarcinoma cell line) cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Secondary
All lanes : HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution
Predicted band size: 85 kDa -
Paraffin-embedded human kidney tissue stained for Cytosolic Phospholipase A2 using ab239730 at 30 μg/ml in immunohistochemical analysis. DAB staining.
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Anti-Cytosolic Phospholipase A2 antibody [C1] (ab239730) at 5 µg/ml + Recombinant human Cytosolic Phospholipase A2 protein
Predicted band size: 85 kDa -
Paraffin-embedded human pancreas tissue stained for Cytosolic Phospholipase A2 using ab239730 at 30 μg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human stomach tissue stained for Cytosolic Phospholipase A2 using ab239730 at 30 μg/ml in immunohistochemical analysis. DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab239730 has been referenced in 1 publication.
- Li J et al. A novel biomimetic nanomedicine system with anti-inflammatory and anti-osteoporosis effects improves the therapy efficacy of steroid-resistant nephrotic syndrome. J Nanobiotechnology 19:417 (2021). PubMed: 34903236