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Immunology Innate Immunity Macrophage / Inflamm.
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Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246)

  • Datasheet
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Western blot - Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246)
  • Western blot - Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246)

Key features and details

  • Rabbit polyclonal to Cytosolic Phospholipase A2 - N-terminal
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant human Cytosolic Phospholipase A2 protein (ab198469)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Cytosolic Phospholipase A2 antibody - N-terminal
    See all Cytosolic Phospholipase A2 primary antibodies
  • Description

    Rabbit polyclonal to Cytosolic Phospholipase A2 - N-terminal
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment within Human Cytosolic Phospholipase A2 (N terminal). The exact sequence is proprietary.
    Database link: P47712

  • Positive control

    • IHC-P: Mouse duodenum and liver tissues; U87 xenograft tissue. WB: NIH/3T3 and Raji whole cell lysates. ICC/IF: HeLa cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • PLA
    • Cardiovascular
    • Atherosclerosis
    • Lipoprotein metabolism
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipoprotein metabolism
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Raji whole cell lysate (ab30124)
    • NIH/3T3 whole cell lysate (ab7179)
  • Recombinant Protein

    • Recombinant human Cytosolic Phospholipase A2 protein (ab198469)
  • Related Products

    • Recombinant human Cytosolic Phospholipase A2 protein (ab198469)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab227246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Mouse
Human
WB
Mouse
Human
All applications
Rat
Rabbit
Cow
Application Abreviews Notes
ICC/IF
1/100 - 1/1000.
IHC-P
1/100 - 1/1000.
WB
1/500 - 1/3000. Predicted molecular weight: 85 kDa.
Notes
ICC/IF
1/100 - 1/1000.
IHC-P
1/100 - 1/1000.
WB
1/500 - 1/3000. Predicted molecular weight: 85 kDa.

Target

  • Function

    Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.
  • Tissue specificity

    Expressed in various tissues such as macrophages, platelets, neutrophils, fibroblasts and lung endothelium.
  • Sequence similarities

    Contains 1 C2 domain.
    Contains 1 PLA2c domain.
  • Domain

    The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca(2+).
  • Post-translational
    modifications

    Activated by phosphorylation at both Ser-505 and Ser-727.
  • Cellular localization

    Cytoplasm. Cytoplasmic vesicle. Translocates to membrane vesicles in a calcium-dependent fashion.
  • Target information above from: UniProt accession P47712 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 525072 Cow
    • Entrez Gene: 151056 Human
    • Entrez Gene: 5321 Human
    • Entrez Gene: 18783 Mouse
    • Entrez Gene: 665270 Mouse
    • Entrez Gene: 100008748 Rabbit
    • Entrez Gene: 192259 Rat
    • Omim: 600522 Human
    • SwissProt: A4IFJ5 Cow
    • SwissProt: P47712 Human
    • SwissProt: Q6P1J6 Human
    • SwissProt: P47713 Mouse
    • SwissProt: Q3TTY0 Mouse
    • SwissProt: Q9TT38 Rabbit
    • SwissProt: O54728 Rat
    • SwissProt: P50393 Rat
    • Unigene: 444933 Human
    • Unigene: 497200 Human
    • Unigene: 160067 Mouse
    • Unigene: 4186 Mouse
    • Unigene: 10162 Rat
    • Unigene: 91079 Rat
    see all
  • Alternative names

    • Calcium dependent phospholipid binding protein antibody
    • CPLA 2 antibody
    • cPLA2 alpha antibody
    • cPLA2 antibody
    • Cytosolic phospholipase A2 antibody
    • Cytosolic phospholipase A2 group IVA antibody
    • Lysophospholipase antibody
    • MGC126350 antibody
    • PA24A_HUMAN antibody
    • Phosphatidylcholine 2 acylhydrolase antibody
    • Phosphatidylcholine 2-acylhydrolase antibody
    • Phospholipase A2 group 4 A antibody
    • Phospholipase A2 group IVA (cytosolic calcium dependent) antibody
    • Phospholipase A2 group IVA antibody
    • PhospholipaseA2 antibody
    • PLA2G4 antibody
    • pla2g4a antibody
    see all

Images

  • Western blot - Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246)
    Western blot - Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246)
    Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246) at 1/1000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

    Predicted band size: 85 kDa



    7.5% SDS-PAGE gel.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246)

    Paraffin-embedded mouse duodenum tissue stained for Cytosolic Phospholipase A2 using ab227246 at 1/500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246)

    Paraffin-embedded mouse liver tissue stained for Cytosolic Phospholipase A2 using ab227246 at 1/500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246)

    Paraffin-embedded U87 xenograft tissue stained for Cytosolic Phospholipase A2 using ab227246 at 1/500 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246)

    Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Cytosolic Phospholipase A2 (green) using ab227246 at 1/200 dilution in ICC/IF.

    Lower panel: Merged with DNA probe.

  • Western blot - Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246)
    Western blot - Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246)
    Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg

    Predicted band size: 85 kDa



    7.5% SDS-PAGE gel.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab227246? Please let us know so that we can cite the reference in this datasheet.

    ab227246 has not yet been referenced specifically in any publications.

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