Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246)
Key features and details
- Rabbit polyclonal to Cytosolic Phospholipase A2 - N-terminal
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Cytosolic Phospholipase A2 antibody - N-terminal
See all Cytosolic Phospholipase A2 primary antibodies -
Description
Rabbit polyclonal to Cytosolic Phospholipase A2 - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P MouseHumanWB MouseHuman
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Immunogen
Recombinant fragment within Human Cytosolic Phospholipase A2 (N terminal). The exact sequence is proprietary.
Database link: P47712 -
Positive control
- IHC-P: Mouse duodenum and liver tissues; U87 xenograft tissue. WB: NIH/3T3 and Raji whole cell lysates. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Mouse
Human
|
| WB |
Mouse
Human
|
| All applications |
Rat
Rabbit
Cow
|
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100 - 1/1000.
|
|
| IHC-P |
1/100 - 1/1000.
|
|
| WB |
1/500 - 1/3000. Predicted molecular weight: 85 kDa.
|
| Notes |
|---|
|
ICC/IF
1/100 - 1/1000. |
|
IHC-P
1/100 - 1/1000. |
|
WB
1/500 - 1/3000. Predicted molecular weight: 85 kDa. |
Target
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Function
Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response. -
Tissue specificity
Expressed in various tissues such as macrophages, platelets, neutrophils, fibroblasts and lung endothelium. -
Sequence similarities
Contains 1 C2 domain.
Contains 1 PLA2c domain. -
Domain
The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca(2+). -
Post-translational
modificationsActivated by phosphorylation at both Ser-505 and Ser-727. -
Cellular localization
Cytoplasm. Cytoplasmic vesicle. Translocates to membrane vesicles in a calcium-dependent fashion. - Information by UniProt
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Database links
- Entrez Gene: 525072 Cow
- Entrez Gene: 151056 Human
- Entrez Gene: 5321 Human
- Entrez Gene: 18783 Mouse
- Entrez Gene: 665270 Mouse
- Entrez Gene: 100008748 Rabbit
- Entrez Gene: 192259 Rat
- Omim: 600522 Human
see all -
Alternative names
- Calcium dependent phospholipid binding protein antibody
- CPLA 2 antibody
- cPLA2 alpha antibody
see all
Images
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Western blot - Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246)Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246) at 1/1000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg
Predicted band size: 85 kDa7.5% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246)Paraffin-embedded mouse duodenum tissue stained for Cytosolic Phospholipase A2 using ab227246 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246)Paraffin-embedded mouse liver tissue stained for Cytosolic Phospholipase A2 using ab227246 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246)Paraffin-embedded U87 xenograft tissue stained for Cytosolic Phospholipase A2 using ab227246 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246)Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Cytosolic Phospholipase A2 (green) using ab227246 at 1/200 dilution in ICC/IF.
Lower panel: Merged with DNA probe.
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Western blot - Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246)Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg
Predicted band size: 85 kDa7.5% SDS-PAGE gel.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab227246 has not yet been referenced specifically in any publications.
