Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246)
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Overview
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Product name
Anti-Cytosolic Phospholipase A2 antibody - N-terminal
See all Cytosolic Phospholipase A2 primary antibodies -
Description
Rabbit polyclonal to Cytosolic Phospholipase A2 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Cow
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Immunogen
Recombinant fragment within Human Cytosolic Phospholipase A2 (N terminal). The exact sequence is proprietary.
Database link: P47712 -
Positive control
- IHC-P: Mouse duodenum and liver tissues; U87 xenograft tissue. WB: NIH/3T3 and Raji whole cell lysates. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab227246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/1000. | |
| IHC-P | 1/100 - 1/1000. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 85 kDa. |
Target
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Function
Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response. -
Tissue specificity
Expressed in various tissues such as macrophages, platelets, neutrophils, fibroblasts and lung endothelium. -
Sequence similarities
Contains 1 C2 domain.
Contains 1 PLA2c domain. -
Domain
The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca(2+). -
Post-translational
modificationsActivated by phosphorylation at both Ser-505 and Ser-727. -
Cellular localization
Cytoplasm. Cytoplasmic vesicle. Translocates to membrane vesicles in a calcium-dependent fashion. - Information by UniProt
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Database links
- Entrez Gene: 525072 Cow
- Entrez Gene: 151056 Human
- Entrez Gene: 5321 Human
- Entrez Gene: 18783 Mouse
- Entrez Gene: 665270 Mouse
- Entrez Gene: 100008748 Rabbit
- Entrez Gene: 192259 Rat
- Omim: 600522 Human
see all -
Alternative names
- Calcium dependent phospholipid binding protein antibody
- CPLA 2 antibody
- cPLA2 alpha antibody
see all
Images
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Western blot - Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246)Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246) at 1/1000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg
Predicted band size: 85 kDa7.5% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246)Paraffin-embedded mouse duodenum tissue stained for Cytosolic Phospholipase A2 using ab227246 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246)Paraffin-embedded mouse liver tissue stained for Cytosolic Phospholipase A2 using ab227246 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246)Paraffin-embedded U87 xenograft tissue stained for Cytosolic Phospholipase A2 using ab227246 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246)Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Cytosolic Phospholipase A2 (green) using ab227246 at 1/200 dilution in ICC/IF.
Lower panel: Merged with DNA probe.
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Western blot - Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246)Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg
Predicted band size: 85 kDa7.5% SDS-PAGE gel.
References
ab227246 has not yet been referenced specifically in any publications.
