Cytosolic Phospholipase A2 Assay Kit (ab133090)

Submit a review Q&A (3)References (8)

Key features and details

  • Assay type: Enzyme activity (quantitative)
  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Sample type: Cell culture extracts, Tissue Extracts

Overview

  • Product name

    Cytosolic Phospholipase A2 Assay Kit
    See all Cytosolic Phospholipase A2 kits

  • Detection method

    Colorimetric

  • Sample type

    Cell culture extracts, Tissue Extracts

  • Assay type

    Enzyme activity (quantitative)

  • Species reactivity

    Reacts with: Mammals, Other species

  • Product overview

    Cytosolic Phospholipase A2 Assay Kit (ab133090) can be used to determine the activity of cytosolic Phospholipase A2 (cPLA2) in purified preparations, cell cultures, or tissue homogenates. Use of this assay with preparations containing more than one type of PLA2 will result in the measurement of total PLA2 activity rather than cPLA2 alone.

    If you want to detect secretory PLA2, we recommend using Secretory Phospholipase Assay Kit (ab133089).

  • Notes

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Microplate reader

Properties

Associated products

    References (8)

    Publishing research using ab133090? Please let us know so that we can cite the reference in this datasheet.

    ab133090 has been referenced in 8 publications.

    • Janks L  et al. ATP-Gated P2X7 Receptors Require Chloride Channels To Promote Inflammation in Human Macrophages. J Immunol 202:883-898 (2019). PubMed: 30598517
    • Horváth Á  et al. Subantimicrobial Dose Doxycycline Worsens Chronic Arthritis-Induced Bone Microarchitectural Alterations in a Mouse Model: Role of Matrix Metalloproteinases? Front Pharmacol 10:233 (2019). PubMed: 30949048
    • Ghosh S  et al. TNFa mediated ceramide generation triggers cisplatin induced apoptosis in B16F10 melanoma in a PKCd independent manner. Oncotarget 9:37627-37646 (2018). PubMed: 30701020
    • Lim JA  et al. Therapeutic Benefit of Autophagy Modulation in Pompe Disease. Mol Ther 26:1783-1796 (2018). PubMed: 29804932
    • Tamayose CI  et al. Non-Clinical Studies for Evaluation of 8-C-Rhamnosyl Apigenin Purified from Peperomia obtusifolia against Acute Edema. Int J Mol Sci 18:N/A (2017). PubMed: 28906474
    • Bhattacharjee A  et al. Leishmania donovani-Induced Prostaglandin E2 Generation Is Critically Dependent on Host Toll-Like Receptor 2-Cytosolic Phospholipase A2 Signaling. Infect Immun 84:2963-73 (2016). PubMed: 27481248
    • Kirac E  et al. Analysis of polyunsaturated fatty acids and the omega-6 inflammatory pathway in hepatic ischemia/re-perfusion injury. Mol Med Rep 12:4149-56 (2015). Functional Studies ; Rat . PubMed: 26062838
    • Aslan M  et al. Inhibition of neutral sphingomyelinase decreases arachidonic acid mediated inflammation in liver ischemia-reperfusion injury. Int J Clin Exp Pathol 7:7814-23 (2014). PubMed: 25550821

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Question

    Should I warm up components to room temperature before use? I do not have compressed Nitrogen, do you have any other suggestions for evaporating the ethanol in the substrate?

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    Answer

    Since this is an enzymatic assays, the kit components should not be ice cold when it is performed. However, the DNTB should be kept on ice. If you do not have access to dry nitrogen gas, you can use a vacuum centrifuge to evaporate the ethanol from the substrate.

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    Question

    Dear Supplier,

    We would like to help our customer by ordering them your product. For regulatory purposes we need to know the volume ethanol content of the kit performed according to European legislation in advance.could you please provide the data for this product.

    Thank you in advance.

    Regards,

    Read More
    Answer

    Thank you for contacting us and your interest in our products.

    I can confirm that the Cytosolic Phospholipase A2 Assay Kit (ab133090), does contain ethanol. There are 2 vials of Arachidonoyl Thio-PC (Substrate). Each vial contains 10 ml of ethanol. There is therefore a total of 20 mL of ethanol in the kit.

    I hope this information has been of help. Please feel free to contact me with future technical questions.

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    Question

    Dear Sir/Madam,








    I am Dr Christina Kanonidou and I am interested in an assay kit for the determination of Lp-PLA2 (PAF-AH) activity in plasma/serum for a research protocol.

    I would be grateful if you could inform me whether you have such a kit available and if yes, could you please forward the relevant prospectous to me?

    It would also be much appreciated if you could inform me about the cost of it and if there is a distributor of your products in Greece.



    Thank you in advance.



    With kind regards,

    Read More
    Answer

    Thank you for your inquiry.

    I am sorry to confirm that we do not offer a kit for the measurement of the activity specifically of Lp-PLA2 (phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) = Lipoprotein-associated phospholipase A2 = PLA2G7).

    Cytosolic Phospholipase A2 Assay Kit (ab133090) can be used to determine the activity of cytosolic Phospholipase A2 (cPLA2, PLA2G4 = Phospholipase A2, group IVA (cytosolic, calcium-dependent)) in purified preparations, cell cultures, or tissue homogenates.
    Use of this assay with preparations containing more than one type of PLA2 will result in the measurement of total PLA2 activity rather than cPLA2 alone.

    Maybe this kit would be suitable for you?

    Please review the datasheet to confirm that this kit would be suitable for your experiments.

    https://www.abcam.com/Cytosolic-Phospholipase-A2-Assay-Kit-ab133090.html (or use the following: https://www.abcam.com/Cytosolic-Phospholipase-A2-Assay-Kit-ab133090.html).

    I am happy to confirm that we sell directly to Greece. Please set your location on our homepage to Greece (in the right top corner). This will provide country specific information about prices and shipping, etc.

    I hope this information is helpful and wish you good luck with your experiments.

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