Anti-Cytosolic Phospholipase A2 (phospho S505) antibody (ab53105)

Overview

  • Product name
    Anti-Cytosolic Phospholipase A2 (phospho S505) antibody
    See all Cytosolic Phospholipase A2 primary antibodies
  • Description
    Rabbit polyclonal to Cytosolic Phospholipase A2 (phospho S505)
  • Host species
    Rabbit
  • Specificity
    ab53105 detects endogenous levels of Cytosolic Phospholipase A2 only when phosphorylated at serine 505.
  • Tested applications
    Suitable for: IHC-Fr, WB, IHC-P, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Cytosolic Phospholipase A2 (phospho S505).
    Sequence:

    P-L-Sp-P-L

  • Positive control
    • HIC-P: Human colon carcinoma tissue WB: Extracts from HeLa cells treated with TNF alpha

Properties

Applications

Our Abpromise guarantee covers the use of ab53105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100. PubMed: 23773289
WB 1/300 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 85 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.
  • Tissue specificity
    Expressed in various tissues such as macrophages, platelets, neutrophils, fibroblasts and lung endothelium.
  • Sequence similarities
    Contains 1 C2 domain.
    Contains 1 PLA2c domain.
  • Domain
    The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca(2+).
  • Post-translational
    modifications
    Activated by phosphorylation at both Ser-505 and Ser-727.
  • Cellular localization
    Cytoplasm. Cytoplasmic vesicle. Translocates to membrane vesicles in a calcium-dependent fashion.
  • Information by UniProt
  • Database links
  • Alternative names
    • Calcium dependent phospholipid binding protein antibody
    • CPLA 2 antibody
    • cPLA2 alpha antibody
    • cPLA2 antibody
    • Cytosolic phospholipase A2 antibody
    • Cytosolic phospholipase A2 group IVA antibody
    • Lysophospholipase antibody
    • MGC126350 antibody
    • PA24A_HUMAN antibody
    • Phosphatidylcholine 2 acylhydrolase antibody
    • Phosphatidylcholine 2-acylhydrolase antibody
    • Phospholipase A2 group 4 A antibody
    • Phospholipase A2 group IVA (cytosolic calcium dependent) antibody
    • Phospholipase A2 group IVA antibody
    • PhospholipaseA2 antibody
    • PLA2G4 antibody
    • pla2g4a antibody
    see all

Images

  • All lanes : Anti-Cytosolic Phospholipase A2 (phospho S505) antibody (ab53105) at 1/300 dilution

    Lane 1 : extracts from HeLa cells treated
    with TNF-a (20ng/ml, 30mins)
    Lane 2 : extracts from HeLa cells treated
    with TNF-a (20ng/ml, 30mins) with immunising peptide

    Predicted band size: 85 kDa

  • ab53105 at 1/50 dilution staining Cytosolic Phospholipase A2 in human colon carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.

  • ICC/IF image of ab53105 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53105, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Chao H  et al. Activation of bradykinin B2 receptor induced the inflammatory responses of cytosolic phospholipase A2 after the early traumatic brain injury. Biochim Biophys Acta N/A:N/A (2018). WB . Read more (PubMed: 29894755) »
  • Hall Z  et al. Myc Expression Drives Aberrant Lipid Metabolism in Lung Cancer. Cancer Res 76:4608-18 (2016). IHC-P ; Human . Read more (PubMed: 27335109) »

See all 9 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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