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    cytosolparticulate-rapid-separation-kit-ab65398.pdf

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Cytosol/Particulate Rapid Separation Kit (ab65398)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (1)References (2)

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Key features and details

  • Assay type: Direct
  • Assay time: 1 hr
  • Sample type: Adherent cells, Suspension cells, Tissue

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Overview

  • Product name

    Cytosol/Particulate Rapid Separation Kit
  • Sample type

    Tissue, Adherent cells, Suspension cells
  • Assay type

    Direct
  • Assay time

    1h 00m
  • Product overview

    Abcam's Cytosol/Particulate Rapid Separation Kit physically separate cytosol from particulate compartments rapidly through an oil layer and thus the two fractions would not contact or diffuse to each other. Using the method, contaminations can be avoided even for small molecules. Subcellular localization and analyses of factors interested can be performed accurately.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K267 Cytosol/Particulate Separation Kit. K267-50 is the same size as the 50 test size of ab65398.

    The location and translocation of proteins, signaling molecules, and other small molecules inside cells regulate cell growth, differentiation and many other cellular functions. Separating cytosol and particulate fractions is an important step in studying subcellular localization of cellular components. However, traditional methods usually take hours to separate cytosol from particulates, so that some cellular components especially small molecules and metabolites are getting diffused or redistributed during the separation procedure.

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 50 tests
    Cell Suspension Buffer Red 1 x 2ml
    Cytosol Releasing Buffer Green 1 x 2ml
    Oil Layer WM 1 x 25ml
    Particulate Layer Blue 1 x 2ml
  • Research areas

    • Kits/ Lysates/ Other
    • Kits
    • Molecular Biology Kits
    • Cell Fractionation Kits
    • Kits/ Lysates/ Other
    • Kits
    • Extraction Kit
    • Cytosol
  • Relevance

    Cytosol/Particulate separation is the method for separating cytosol and particulate fractions. The location and translocation of proteins, signaling molecules, and other small molecules inside cells regulate cell growth, differentiation and many other cellular functions. Separating cytosol and particulate fractions is an important step in studying subcellular localization of cellular components.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2)

Publishing research using ab65398? Please let us know so that we can cite the reference in this datasheet.

ab65398 has been referenced in 2 publications.

  • Pannecoucke E  et al. Cell-penetrating Alphabody protein scaffolds for intracellular drug targeting. Sci Adv 7:N/A (2021). PubMed: 33771865
  • Fuentelsaz-Romero S  et al. GM-CSF Expression and Macrophage Polarization in Joints of Undifferentiated Arthritis Patients Evolving to Rheumatoid Arthritis or Psoriatic Arthritis. Front Immunol 11:613975 (2020). PubMed: 33679701

Customer reviews and Q&As

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Question

hi
I wonder whether you can decribe what is the exactly particulate fraction? is it including plasmmamembrane?
thanks.
Wei

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Abcam community

Verified customer

Asked on Nov 22 2012

Answer

Thank you for contacting us.

Particulate fractions are the unsoluble fractions of lysaed cells e.g. plasma membrane. You are correct!

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Abcam Scientific Support

Answered on Nov 22 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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