Cytosol/Particulate Rapid Separation Kit (ab65398)
- Datasheet
- References
- Protocols
Overview
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Product name
Cytosol/Particulate Rapid Separation Kit -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Direct -
Assay time
1h 00m -
Product overview
Abcam's Cytosol/Particulate Rapid Separation Kit physically separate cytosol from particulate compartments rapidly through an oil layer and thus the two fractions would not contact or diffuse to each other. Using the method, contaminations can be avoided even for small molecules. Subcellular localization and analyses of factors interested can be performed accurately.
Visit our FAQs page for tips and troubleshooting. -
Notes
The location and translocation of proteins, signaling molecules, and other small molecules inside cells regulate cell growth, differentiation and many other cellular functions. Separating cytosol and particulate fractions is an important step in studying subcellular localization of cellular components. However, traditional methods usually take hours to separate cytosol from particulates, so that some cellular components especially small molecules and metabolites are getting diffused or redistributed during the separation procedure.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 50 tests Cell Suspension Buffer Red 1 x 2ml Cytosol Releasing Buffer Green 1 x 2ml Oil Layer WM 1 x 25ml Particulate Layer Blue 1 x 2ml -
Research areas
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Relevance
Cytosol/Particulate separation is the method for separating cytosol and particulate fractions. The location and translocation of proteins, signaling molecules, and other small molecules inside cells regulate cell growth, differentiation and many other cellular functions. Separating cytosol and particulate fractions is an important step in studying subcellular localization of cellular components.
Protocols
References
ab65398 has not yet been referenced specifically in any publications.
