Key features and details
- Assay type: Direct
- Assay time: 1 hr
- Sample type: Adherent cells, Suspension cells, Tissue
Product nameCytosol/Particulate Rapid Separation Kit
Sample typeTissue, Adherent cells, Suspension cells
Assay time1h 00m
Abcam's Cytosol/Particulate Rapid Separation Kit physically separate cytosol from particulate compartments rapidly through an oil layer and thus the two fractions would not contact or diffuse to each other. Using the method, contaminations can be avoided even for small molecules. Subcellular localization and analyses of factors interested can be performed accurately.
Visit our FAQs page for tips and troubleshooting.
This product is manufactured by BioVision, an Abcam company and was previously called K267 Cytosol/Particulate Separation Kit. K267-50 is the same size as the 50 test size of ab65398.
The location and translocation of proteins, signaling molecules, and other small molecules inside cells regulate cell growth, differentiation and many other cellular functions. Separating cytosol and particulate fractions is an important step in studying subcellular localization of cellular components. However, traditional methods usually take hours to separate cytosol from particulates, so that some cellular components especially small molecules and metabolites are getting diffused or redistributed during the separation procedure.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 50 tests Cell Suspension Buffer Red 1 x 2ml Cytosol Releasing Buffer Green 1 x 2ml Oil Layer WM 1 x 25ml Particulate Layer Blue 1 x 2ml
RelevanceCytosol/Particulate separation is the method for separating cytosol and particulate fractions. The location and translocation of proteins, signaling molecules, and other small molecules inside cells regulate cell growth, differentiation and many other cellular functions. Separating cytosol and particulate fractions is an important step in studying subcellular localization of cellular components.
ab65398 has been referenced in 2 publications.
- Pannecoucke E et al. Cell-penetrating Alphabody protein scaffolds for intracellular drug targeting. Sci Adv 7:N/A (2021). PubMed: 33771865
- Fuentelsaz-Romero S et al. GM-CSF Expression and Macrophage Polarization in Joints of Undifferentiated Arthritis Patients Evolving to Rheumatoid Arthritis or Psoriatic Arthritis. Front Immunol 11:613975 (2020). PubMed: 33679701