Cytosol/Particulate Rapid Separation Kit (ab65398)

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Overview

  • Product name
    Cytosol/Particulate Rapid Separation Kit
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay type
    Direct
  • Assay time
    1h 00m
  • Product overview

    Abcam's Cytosol/Particulate Rapid Separation Kit physically separate cytosol from particulate compartments rapidly through an oil layer and thus the two fractions would not contact or diffuse to each other. Using the method, contaminations can be avoided even for small molecules. Subcellular localization and analyses of factors interested can be performed accurately.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    The location and translocation of proteins, signaling molecules, and other small molecules inside cells regulate cell growth, differentiation and many other cellular functions. Separating cytosol and particulate fractions is an important step in studying subcellular localization of cellular components. However, traditional methods usually take hours to separate cytosol from particulates, so that some cellular components especially small molecules and metabolites are getting diffused or redistributed during the separation procedure.

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 50 tests
    Cell Suspension Buffer Red 1 x 2ml
    Cytosol Releasing Buffer Green 1 x 2ml
    Oil Layer WM 1 x 25ml
    Particulate Layer Blue 1 x 2ml
  • Research areas
  • Relevance
    Cytosol/Particulate separation is the method for separating cytosol and particulate fractions. The location and translocation of proteins, signaling molecules, and other small molecules inside cells regulate cell growth, differentiation and many other cellular functions. Separating cytosol and particulate fractions is an important step in studying subcellular localization of cellular components.

Protocols

References

ab65398 has not yet been referenced specifically in any publications.

Publishing research using ab65398? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1 Review or Q&A

10
Votes: Highest First

hi
I wonder whether you can decribe what is the exactly particulate fraction? is it including plasmmamembrane?
thanks.
Wei

Thank you for contacting us.

Particulate fractions are the unsoluble fractions of lysaed cells e.g. plasma membrane. You are correct!

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