• Product name

    CyTRAK Orange™ 50µl (5 mM)
  • Tested applications

    Suitable for: FM, Flow Cyt, ICC/IFmore details
  • General notes

    CyTRAK Orange™ is a novel orange fluorescent dye [related to DRAQ5™ (ab108410)] that stains both nucleus and cytoplasm. It is water soluble and membrane permeant which can be used in live and fixed cells in combination with other common fluorophores, such as GFP-fusions, green-labelled antibodies (FITC or DyLight® 488) and far-red dyes. CyTRAK Orange™ preferentially stains the nucleus, but also defines the cytoplasmic area in both live and fixed preparations. CyTRAK Orange™ does the work of two fluorophores! Key features of CyTRAK Orange™ include:

    • Rapid staining of dsDNA/nuclei of LIVE or fixed cells
    • Differential intensity of DNA binding and Cytoplasmic labelling - One fluorophore doing the work of two
    • Low photobleaching effect
    • Ideal for use with GFP, FITC & DyLight® 488 and 649 labels
    • No lyse / no wash protocols
    • It is membrane-permeant


    • 457, 488 & 549 (Exmax 515nm)
    • similar profile to Propidium Iodide
    • co-excited with FITC/Cy2/eGFP and PE-Cy7
    • NOT excited by UV or red lasers (e.g. 633 & 647 nm)

    Emission (instrument dependent):

    • Emmax 615 nm
    • No significant spectral overlap with co-excited green fluors (eGFP, FITC, Cy2)
    • Visualize GFP-tagged temporospatial changes in live cells in a single acquisition from a 488 nm excitation



Our Abpromise guarantee covers the use of ab109203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FM Use at an assay dependent concentration.
Flow Cyt 1/1000 - 1/300. For Nucleated Cell Gate = 5µM; For Discrimination of Arrested/ Senescent Cells = 15µM
ICC/IF 1/1000. For microscopy counterstain = 5µM
It is highly recommended that the concentration and labelling conditions are carefully determined by each investigator for optimal performance in the assay of interest. For more specific information about the applications, please refer to the Protocol Booklet.


  • Live cells expressing eGFP/CyclinB1 were labelled with CyTRAK Orange™ (end concentration 20µM) for 20 minutes at 37 C, no wash. Image was captured using confocal microscopy at 488nm simultaneously: i) CyTRAK Orange™ channel - 598/40nm; ii) eGFP/CyclinB1 channel - 522/35nm



ab109203 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A


Je vous ai envoyé de nouveau le devis ****.

Par précaution je vous envoie en pièce jointe le pdf de ce même devis.

J'espère que vous ferez bonne réception de ce document. Vérifiez votre dossier "courrier indésirable", le devis s'y est peut-être glissé.

N'hésitez pas à me contacter de nouveau si vous avez d'autres questions.

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Merci pour votre réponse.

Un devis vous a été envoyé dans un autre email. La référence du devis est *****.

N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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Merci de nous avoir contactés.

Nous avons récemment ajouté à notre catalogue une gamme de produits de marquage fluorescentappelés kits CytoPainter qui permettent de marquer les organites tels que les mitochondries, lysosomes et filaments d'actine.
Le lien vers la liste de ces kits est : https://www.abcam.com/CytoPainter.

Merci de m'indiquer si vous désirez ajouter un de ces produits au devis que vous m'avez demandé pour le DRAQ5 et CyTRAQ Orange.

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Thank you for your enquiry. This CyTRAK Orange (ab109203) is a novel orange fluorescent dye [related to DRAQ5™ (ab108410)] that stains both nucleus and cytoplasm. Please rest assured that it too can be used in live cell imaging. However, we have not tested if there are any effect for experiments up to 96 hr but do not foresee any complications. I would suggest that you also run a positive control (without CyTRAK) to confirm any results you get. I hope I have answered your questions. If there is anything else that I can help you with please do not hesitate to contact me. Have a nice day!

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