D 2 Hydroxyglutarate Assay Kit (Colorimetric) (ab211070)

Overview

  • Product name

    D 2 Hydroxyglutarate Assay Kit (Colorimetric)
  • Detection method

    Colorimetric
  • Sample type

    Urine, Cell Lysate, Tissue Lysate
  • Assay type

    Quantitative
  • Sensitivity

    10 µM
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    D-2-Hydroxyglutarate Assay Kit (ab211070) provides a convenient method to detect D-2-Hydroxyglutarate (D2HG) in urine, cell and tissue lysates. In this assay, D-2-Hydroxyglutarate is oxidized to α-Ketoglutarate in the presence of D2HG Enzyme and Substrate Mix. The intermediate reduces the probe to a colored product with strong absorbance at OD 450 nm. This absorbance is proportional to the amount of D2HG present in the samples. This assay kit is fast, sensitive, easy to use and high-through adaptable. It can measure D-2-Hydroxyglutarate levels less than 10 µM in various samples. This product is specific for D-2-Hydroxyglutarate and does not detect L-2-Hydroxyglutarate.

  • Notes

    In eukaryotic cells, Isocitrate Dehydrogenase (IDH1, IDH2 and IDH3) catalyzes the interconversion of Isocitrate and α-Ketoglutarate. In human cancers, an IDH mutation causes a gain-of-function, which reduces its affinity for isocitrate and facilitates the conversion of αketoglutarate to D-2-Hydroxyglutarate in the presence of NADP. D-2-Hydroxyglutarate (D2HG, D-2-Hydroxyglutarate) is present at low level in normal cells and tissues, but is significantly elevated in metabolic diseases and various cancers. Therefore, detection of elevated D2HG is important for early diagnosis, prognosis and the development of therapeutic strategies against these maladies.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    D2HG Assay Buffer 1 x 20ml
    D2HG Enzyme 1 vial
    D2HG Substrate Mix 1 vial
    D2HG Standard 1 vial

Images

  • Measurement of D-2-Hydroxyglutarate in different cell lysates: 3T3 (80 µg; mouse), Jurkat (12 µg), HeLa (15 µg), MDA-MB-468 (6 µg), and HepG2 (2 μg).

  • Measurement of D-2-Hydroxyglutarate in rat liver lysate (120 µg) and rat kidney lysate (240 µg).

     

Protocols

References

ab211070 has not yet been referenced specifically in any publications.

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