Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7555] to D Amino Acid Oxidase
- Suitable for: WB
- Reacts with: Human
Product nameAnti-D Amino Acid Oxidase antibody [EPR7555]
See all D Amino Acid Oxidase primary antibodies
DescriptionRabbit monoclonal [EPR7555] to D Amino Acid Oxidase
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Human
Synthetic peptide within Human D Amino Acid Oxidase aa 250-350. The exact sequence is proprietary.
- Recombinant Human D Amino Acid Oxidase protein (ab117193) can be used as a positive control in WB. Human fetal kidney lysate; SH-SY5Y cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab140127 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 39 kDa.|
FunctionRegulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids.
Sequence similaritiesBelongs to the DAMOX/DASOX family.
- Information by UniProt
- D-amino-acid oxidase antibody
- DAAO antibody
- DAMOX antibody
All lanes : Anti-D Amino Acid Oxidase antibody [EPR7555] (ab140127) at 1/1000 dilution (purified)
Lane 1 : Human fetal kidney lysates
Lane 2 : Human cerebellum lysates
Lysates/proteins at 15 µg per lane.
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 39 kDa
Blocking and diluting buffer: 5% NFDM/TBST
Anti-D Amino Acid Oxidase antibody [EPR7555] (ab140127) at 1/1000 dilution (unpurified) + Human fetal kidney lysate at 10 µg
goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 39 kDa
Immunofluorescent analysis of SH-SY5Y cells labelling D Amino Acid Oxidase with unpurified ab140127 at 1/100 dilution.
ab140127 has not yet been referenced specifically in any publications.