Recombinant
RabMAb

Recombinant Anti-D Amino Acid Oxidase antibody [EPR7555] (ab140127)

Overview

  • Product name

    Anti-D Amino Acid Oxidase antibody [EPR7555]
    See all D Amino Acid Oxidase primary antibodies
  • Description

    Rabbit monoclonal [EPR7555] to D Amino Acid Oxidase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human D Amino Acid Oxidase aa 250-350. The exact sequence is proprietary.

  • Positive control

    • Recombinant Human D Amino Acid Oxidase protein (ab117193) can be used as a positive control in WB. Human fetal kidney lysate; SH-SY5Y cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab140127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids.
    • Sequence similarities

      Belongs to the DAMOX/DASOX family.
    • Cellular localization

      Peroxisome.
    • Information by UniProt
    • Database links

    • Alternative names

      • D-amino-acid oxidase antibody
      • DAAO antibody
      • DAMOX antibody
      • DAO antibody
      • DAO1 antibody
      • EC 1.4.3.3 antibody
      • MGC35381 antibody
      • OXDA antibody
      • OXDA_HUMAN antibody
      see all

    Images

    • All lanes : Anti-D Amino Acid Oxidase antibody [EPR7555] (ab140127) at 1/1000 dilution (purified)

      Lane 1 : Human fetal kidney lysates
      Lane 2 : Human cerebellum lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size: 39 kDa



      Blocking and diluting buffer: 5% NFDM/TBST

    • Anti-D Amino Acid Oxidase antibody [EPR7555] (ab140127) at 1/1000 dilution (unpurified) + Human fetal kidney lysate at 10 µg

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 39 kDa

    • Immunofluorescent analysis of SH-SY5Y cells labelling D Amino Acid Oxidase with unpurified ab140127 at 1/100 dilution.

    References

    ab140127 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab140127.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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