Key features and details
- NMDA glutamate site antagonist. 1 ml water soluble pack.
- CAS Number: 79055-68-8
- Purity: > 99%
- Soluble in 1 ml of water to give specified mM/ml concentration
- Form / State: Solid
- Source: Synthetic
Product nameD-AP5 (mM/ml), NMDA glutamate site antagonist
DescriptionNMDA glutamate site antagonist. 1 ml water soluble pack.
Competitive NMDA receptor glutamate site antagonist. More active form of DL-AP5.
Soluble in 1 ml water to give specified mM/ml concentration. Find out more.
Chemical nameD-(-)-2-Amino-5-phosphonopentanoic acid
Storage instructionsStore at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in 1 ml of water to give specified mM/ml concentration
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
2D chemical structure image of ab144482, D-AP5 (mM/ml), NMDA glutamate site antagonist
Fractional current mediated by AMPARs increases between birth and hearing onset.
A: Representative mixed glutamatergic responses from P1, P5 and P9 neurons (average of 10 recordings), scaled to peak AMPA current; distinct AMPA and NMDA components visible in each trace. B: Same traces as in A, showing the mixed glutamatergic current, the pharmacologically isolated NMDAR component, and the response after application of AMPAR and NMDAR antagonists, D-APV and CNQX, which abolishes the glutamatergic response. All recordings in Mg++-free ACSF. Note change in scalebars for P9 recordings. C: Ratios of peak AMPA/NMDA current in 39 cells from slices P1-P12. During this period, AMPA/NMDA ratio increases as a function of age (p = 0.012, Kruskal-Wallis; P1/2 vs P9/10, p = 0.048; P7/8 vs P9/10, p = 0.30; P9/10 vs P11/12 p = 0.010; linear regression slope 0.14/day, r2 = 0.32; exponential fit r2 = 0.36). Filled black circles represent means ± SEMs at two-day intervals. Filled gray circles represent cells shown in A,B. D: Increase in AMPA/NMDA ratio with age is accompanied by small increases in average AMPA current (open circles, regressed to gray line) and decreases in average NMDAR current (filled circles, regressed to black line).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab144482 has been referenced in 100 publications.
- Rojas A et al. Activation of group I metabotropic glutamate receptors potentiates heteromeric kainate receptors. Mol Pharmacol 83:106-21 (2013). PubMed: 23066089
- Budisantoso T et al. Evaluation of glutamate concentration transient in the synaptic cleft of the rat calyx of Held. J Physiol 591:219-39 (2013). PubMed: 23070699
- Maroto M et al. Chondroitin sulfate, a major component of the perineuronal net, elicits inward currents, cell depolarization, and calcium transients by acting on AMPA and kainate receptors of hippocampal neurons. J Neurochem : (2013). PubMed: 23350646
- Copits BA & Swanson GT Kainate receptor post-translational modifications differentially regulate association with 4.1N to control activity-dependent receptor endocytosis. J Biol Chem 288:8952-65 (2013). PubMed: 23400781
- Tucker K et al. Glucose sensitivity of mouse olfactory bulb neurons is conveyed by a voltage-gated potassium channel. J Physiol 591:2541-61 (2013). PubMed: 23478133