Overview

  • Product name

    Anti-D Box Binding Protein antibody [EPR22370-51]
    See all D Box Binding Protein primary antibodies
  • Description

    Rabbit monoclonal [EPR22370-51] to D Box Binding Protein
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IF, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human D Box Binding Protein aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q10586

  • Positive control

    • WB: Human hypothalamus and brain lysates; HEK-293T transfected with D Box Binding Protein with a C-terminal myc-His tag. ICC/IF: 293T cells. FC: HEK-293T (transfected with myc-his tagged D Box Binding Protein expression vector) cells. IP: HEK-293T (transfected with myc-His tagged D Box Binding Protein expression vector) cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22370-51
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab222112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 34 kDa).

 

 

Flow Cyt 1/600.

 

 

ICC/IF 1/100.
IP 1/30.

 

 

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      This transcriptional activator recognizes and binds to the sequence 5'-RTTAYGTAAY-3' found in the promoter of genes such as albumin, CYP2A4 and CYP2A5. It is not essential for circadian rhythm generation, but modulates important clock output genes. May be a direct target for regulation by the circadian pacemaker component clock. May affect circadian period and sleep regulation.
    • Tissue specificity

      Ubiquitously expressed. Expressed in the suprachiasmatic nuclei (SCN) and in most peripheral tissues, with a strong circadian rhythmicity.
    • Sequence similarities

      Belongs to the bZIP family. PAR subfamily.
      Contains 1 bZIP domain.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Albumin D box-binding protein antibody
      • Albumin D-element-binding protein antibody
      • D site-binding protein antibody
      • DBP antibody
      • DBP_HUMAN antibody
      • Tax-responsive enhancer element-binding protein 302 antibody
      • TAXREB302 antibody
      • TRANSCRIPTION FACTOR DBP antibody
      see all

    Images

    • All lanes : Anti-D Box Binding Protein antibody [EPR22370-51] (ab222112) at 1/1000 dilution

      Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
      Lane 2 : HEK-293T (human embryonic kidney epithelial cell) whole cell lysate
      Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 4 : Human liver lysate
      Lane 5 : Human hypothalamus lysate
      Lane 6 : Human brain lysate
      Lane 7 : Human testis lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 34 kDa
      Observed band size: 45 kDa
      why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      The DBP protein is ubiquitously expressed but mainly at suprachiasmatic nuclei in the hypothalamus or liver, and fine-tuned by circadian clock. The antibody detects a band around the reported molecular weight of DBP in human hypothalamus and brain tissue lysates. Negative control: testis (PMID: 2331750)

      Exposure time: 3 minutes.

    • All lanes : Anti-D Box Binding Protein antibody [EPR22370-51] (ab222112) at 1/1000 dilution

      Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
      Lane 2 : HEK-293T transfected with D Box Binding Protein (WT) expression vector containing a myc-His-tag®, whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 34 kDa
      Observed band size: 52 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Exposure time: 6 seconds.

    • D Box Binding Protein was immunoprecipitated from 0.35 mg HEK-293T (Human embryonic kidney epithelial cell) transfected with myc-His tagged D Box Binding Protein expression vector, whole cell lysate, with ab222112 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab222112 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

      Lane 1: HEK-293T (Human embryonic kidney epithelial cell) transfected with myc-His tagged D Box Binding Protein expression vector whole cell lysate 10ug.

      Lane 2: ab222112 IP in HEK-293T transfected with myc-His tagged D Box Binding Protein expression vector whole cell lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab222112 in HEK-293T transfected with myc-His tagged D Box Binding Protein expression vector whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 5 seconds.

       

    • Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HEK-293T (Human embryonic kidney epithelial cell) (transfected with myc-his tagged D Box Binding Protein expression vector) cells labelling D Box Binding Protein with ab222112 at 1/600 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Secondary antibody is Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution.

      Cells were stained with rabbit IgG (Left) or ab222112 (Right). Then stained with anti-myc tag conjugated to Alexa Fluor® 488.

    • Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labelling D Box Binding Protein with ab222112 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HEK-293T cells transfected with myc-tagged D Box Binding Protein expression vector. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    References

    ab222112 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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