For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Abcam's PicoProbe D-Lactate Assay Kit (Fluorometric) (ab174096) offers simplicity, sensitivity, and can be adapted to high-throughput research. The assay enzymatically oxidizes D-Lactate generating a fluorescent signal (Ex/Em = 535/587 nm). The signal is directly proportional to the amount of D-Lactate. The assay kit can detect D-Lactate as low as 0.1 µM in a variety of samples.
Visit our FAQs page for tips and troubleshooting.
D-Lactate is the result of anaerobic glycolysis by microorganisms in the gastrointestinal system, and the product of detoxification of methylglyoxal by the glyoxalase system. The presence of abnormal levels of D-Lactate has been linked to a series of pathological conditions, such as diabetes, and appendicitis.
|D-Lactate Assay Buffer||WM||1 x 25ml|
|D-Lactate Enzyme Mix (Lyophilised)||Green||1 vial|
|D-Lactate Standard (100 mM)||Yellow||1 x 0.1ml|
|D-Lactate Substrate Mix (Lyophilised)||Red||1 vial|
|PicoProbe||Blue||1 x 0.4ml|
D-Lactate Standard curve. This assay specifically detected D-Lactate (blue diamonds) but not L-Lactate (red squares). This is example data only.
Diluted samples (1/10, non-diabetic; 1/100 diabetic) were spiked with known amounts of D-Lactate (0-200 pmol) and assayed following kit's protocol. This is example data only.
ab174096 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"