D-Luciferin sodium salt, Chemiluminescent luciferase substrate (ab145164)

Overview

  • Product name

    D-Luciferin sodium salt, Chemiluminescent luciferase substrate
  • Description

    Chemiluminescent luciferase substrate
  • Biological description

    Chemiluminescent luciferase substrate. Water soluble D-Luciferin (ab143654) derivative and D-Luciferin potassium salt (ab143655) analog. Excitation is pH dependent (pH exλ = 327 and 385 nm for pH 4 and pH 11 respectively), emission is pH independent. Emits light on oxidative decarboxylation in the presence of ATP providing a bioluminescent signal for cellular ATP levels in vivo.
  • Purity

    > 99%
  • CAS Number

    103404-75-7
  • Chemical structure

    Chemical Structure

Properties

  • Excitation

    328nm
  • Emission

    328nm
  • Chemical name

    (4S)-2-(6-Oxo-1,3-benzothiazol-2-ylidene)-1,3-thiazolidine-4-carboxylate sodium salt
  • Molecular weight

    302.30
  • Molecular formula

    C11H7N2NaO3S2
  • Storage instructions

    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in water to 25 mM and in DMSO to 25 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    [Na]OC(=O)[C@@H]1N=C(SC1)c2nc3ccc(O)cc3s2
  • Source

    Synthetic

  • Research areas

References

This product has been referenced in:

  • Kuchlyan J  et al. Excited-State Proton Transfer Dynamics of Firefly's Chromophore D-Luciferin in DMSO-Water Binary Mixture. J Phys Chem B 118:13946-53 (2014). Read more (PubMed: 25415652) »
  • McCutcheon DC  et al. Rapid and scalable assembly of firefly luciferase substrates. Org Biomol Chem : (2014). Read more (PubMed: 25525906) »
  • Berger F  et al. Uptake kinetics and biodistribution of 14C-D-luciferin--a radiolabeled substrate for the firefly luciferase catalyzed bioluminescence reaction: impact on bioluminescence based reporter gene imaging. Eur J Nucl Med Mol Imaging 35:2275-85 (2008). Read more (PubMed: 18661130) »

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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