Overview

  • Product name
    D-Mannitol Assay Kit (Colorimetric)
  • Assay type
    Quantitative
  • Sensitivity
    < 10 µM
  • Range
    0 nmol/well - 20 nmol/well
  • Assay time
    0h 20m
  • Species reactivity
    Reacts with: Plants
  • Product overview

    In Abcam's D-Mannitol Assay Kit (Colorimetric) (ab155890), D-mannitol is converted to D-fructose by mannitol dehydrogenase in the presence of NAD to form NADH, which reduces a colorless probe to a chromogen with strong absorbance at 450 nm. This kit can also be used to measure L-arabitol, another sugar alcohol, as L-arabitol acts as substrate for mannitol dehydrogenase. The D-mannitol assay kit is fast (~20 minutes), sensitive and easy to use. This assay kit can detect mannitol level less than 1 nmol/reaction (< 10µM) and can be used for a variety of sample types.

  • Notes

    D-Mannitol is one of the most abundant sugar alcohols in nature. It can be produced in the biosphere by a range of organisms such as bacteria, yeast, fungi and almost all plants. Mannitol functions as a carbohydrate storage depot, as a scavenger for reactive oxygen species and as an osmoprotectant. Recent studies have shown that increased mannitol content in plants protects them against fungal infections and improves resistance to drought and high salinity. The mannitol pathway reversibly converts fructose to mannitol and serves as the main carbohydrate cycle for fungi survival. Measurement of mannitol level is important for evaluating mannitol metabolic pathways and commercially, in developing fungi and drought resistant plants.

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Mannitol Assay Buffer WM 1 x 27ml
    Mannitol Enzyme Mix Green 1 x 30µl
    Mannitol Standard Yellow 1 vial
    Mannitol Substrate Mix Red 1 vial
  • Research areas

Images

  • Mannitol measured in various samples showing quantity (nmol) per mg of extracted protein.

    Protein concentration for samples varied from 2 mg/mL to 7 mg/mL. Samples were diluted 2 fold.

Protocols

References

ab155890 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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