D-Mannitol Assay Kit (Colorimetric) (ab155890)
- Datasheet
- References
- Protocols
Overview
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Product name
D-Mannitol Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Assay type
Quantitative -
Sensitivity
< 10 µM -
Range
0 nmol/well - 20 nmol/well -
Assay time
0h 20m -
Species reactivity
Reacts with: Other species, Mammals -
Product overview
In Abcam's D-Mannitol Assay Kit (Colorimetric) (ab155890), D-mannitol is converted to D-fructose by mannitol dehydrogenase in the presence of NAD to form NADH, which reduces a colorless probe to a chromogen with strong absorbance at 450 nm. This kit can also be used to measure L-arabitol, another sugar alcohol, as L-arabitol acts as substrate for mannitol dehydrogenase. The D-mannitol assay kit is fast (~20 minutes), sensitive and easy to use. This assay kit can detect mannitol level less than 1 nmol/reaction (< 10μM) and can be used for a variety of sample types.
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Notes
D-Mannitol is one of the most abundant sugar alcohols in nature. It can be produced in the biosphere by a range of organisms such as bacteria, yeast, fungi and almost all plants. Mannitol functions as a carbohydrate storage depot, as a scavenger for reactive oxygen species and as an osmoprotectant. Recent studies have shown that increased mannitol content in plants protects them against fungal infections and improves resistance to drought and high salinity. The mannitol pathway reversibly converts fructose to mannitol and serves as the main carbohydrate cycle for fungi survival. Measurement of mannitol level is important for evaluating mannitol metabolic pathways and commercially, in developing fungi and drought resistant plants.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Mannitol Assay Buffer WM 1 x 27ml Mannitol Enzyme Mix Green 1 x 30µl Mannitol Standard Yellow 1 vial Mannitol Substrate Mix Red 1 vial -
Research areas
Images
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Functional Studies - D-Mannitol Assay Kit (Colorimetric) (ab155890)Mannitol measured in various samples showing quantity (nmol) per mg of extracted protein.
Protein concentration for samples varied from 2 mg/mL to 7 mg/mL. Samples were diluted 2 fold.
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Measurement of Mannitol in the extracts of Longyan & LycheMeasurement of Mannitol in the extracts of Longyan & Lyche generated using ab155890
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Mannitol Standard CurveMannitol Standard Curve generated using the method described in the protocol bookelt
Protocols
References
ab155890 has not yet been referenced specifically in any publications.
