Overview

  • Product name

    D-Sorbitol Assay Kit (Colorimetric)
  • Detection method

    Colorimetric
  • Sample type

    Cell Lysate
  • Assay type

    Quantitative
  • Sensitivity

    > 0.1 nmol/well
  • Range

    0.1 nmol/well - 10 nmol/well
  • Assay time

    0h 40m
  • Product overview

    Abcam's D-Sorbitol Assay Kit (Colorimetric) is designed to measure sorbitol in a variety of samples such as foods, fruits, fruit juices, pharmaceuticals, cosmetics, paper and some other biological samples. In the assay, sorbitol is oxidized to fructose with the proportional development of intense color with an absorbance maximum at 560 nm. The assay is useful over the range of 0.1-10 nmol of Sorbitol per sample.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Sorbitol is one of the 6 carbon sugar alcohols. It is commonly used as an artificial sweetener, as a laxative and in cosmetics as a humectant and thickening agent. Sorbitol is produced naturally in a variety of fruits. It can be produced in humans in small amounts by the reduction of glucose by aldose reductase. Due to its poor ability to diffuse across the cell membrane, sorbitol can be trapped in cells and is believed to be one of the causes of damage (due to osmotic effects) in diabetes. Interestingly, sorbitol can be used as a screen for the O154:H7 strain of E. coli, since this strain is one of the few strains which cannot metabolize sorbitol.

  • Platform

    Microplate reader

Properties

Images

  • D-Sorbitol measured in various samples showing nmol/mg (fruits) and nmol/mL (juice).  300 mg (wet weight) of prune and pear was homogenised in 1 mL of water. Juice was diluted in water. Samples were diluted 2-1000 fold.

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Example of standard curve obtained using ab118968.

Protocols

References

ab118968 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for your enquiry.

I can confirm that any publications that we are aware of will be listed on the datasheet. We have a dedicated team who spend time reviewing the literature to find articles that have used our products. However, in many cases, regrettably we do not have access to all the articles mentioning our products, or subscriptions to all the journals that publish them. I'm sorry to confirm there are none to provide on this occasion for ab118968 D-Sorbitol Assay Kit.

Should you have any further questions, please do not hesitate to contact us.

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Answer

Thank you for your enquiry.

I can confirm that any publications that we are aware of will be listed on the datasheet. We have a dedicated team who spend time reviewing the literature to find articles that have used our products.

However, in many cases, regrettably we do not have access to all the articles mentioning our products, or subscriptions to all the journals that publish them.

I'm sorry there are none to provide on this occasion for ab118968 at this moment in time.

We welcome information form customer about our products that have been used in their publications.

Should you have any further questions, please do not hesitate to contact us.

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Answer

Thank you for contacting us. Unfortunatley we do not  know the cause as to why these sample types do not perform in our kit. I have found an assay published at the following link: http://www.sciencedirect.com/science/article/pii/S0009898104005455 Good luck with you experiments. Please do not hesitate to contact us with further questions or if there are other ways in which we might help you reach your research goals.

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Answer

Thank you for contacting us. As it goes with any kit there can be compatible and incompatible samples. We have tested this kit with plasma, serum etc. and knows that it doesn’t work; we do not want our customer to spend time optimizing this kit for such samples.The reason could be that they samples negatively interfere with the kits basic reaction mechanism.  I hope this helps. Please let me know if you have any more questions. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

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