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    d2hgdh-assay-kit-fluorometric-ab239706.pdf

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Signal Transduction Metabolism Mitochondrial
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D2HGDH Assay Kit (Fluorometric) (ab239706)

  • Datasheet
  • SDS
  • Protocol Booklet
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NADH Standard Curve
  • Kinetic measurement of D2HGDH activity in various samples.
  • ) D2HGDH specific activity was calculated in lysates prepared from Rat Liver (5 µg) and Rat Kidney (4 µg)

Key features and details

  • Detection method: Fluorescent
  • Platform: Microplate reader
  • Sample type: Adherent cells, Cell culture supernatant, Suspension cells, Tissue

Overview

  • Product name

    D2HGDH Assay Kit (Fluorometric)
  • Detection method

    Fluorescent
  • Sample type

    Cell culture supernatant, Tissue, Adherent cells, Suspension cells
  • Product overview

    D2HGDH Assay Kit (Fluorometric) (ab239706) provides a quick and easy method for monitoring D2HGDH activity in a wide variety of samples.


    In this assay, D2HGDH converts D-2-Hydroxyglutarate into 2-Oxoglutarate and NADH, which further reduces the probe to generate an intense fluorescent product (Ex/Em = 535/587 nm).


    This kit is simple, sensitive and high-throughput adaptable and can detect as low as 4 µU/µL of D2HGDH activity.


     

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    D2HGDH Assay Buffer 1 x 20ml
    D2HGDH Developer 1 vial
    D2HGDH Positive Control 1 vial
    D2HGDH Substrate 1 x 0.4ml
    NADH Standard 1 vial
    Probe in DMSO 1 x 0.2ml
  • Research areas

    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Antioxidants
  • Relevance

    Catalyzes the oxidation of D-2-hydroxyglutarate to alpha-ketoglutarate. Disease: Defects in D2HGDH are the cause of D-2-hydroxyglutaric aciduria type 1 (D2HGA1) [MIM:600721]. D2HGA1 is a rare recessive neurometabolic disorder causing developmental delay, epilepsy, hypotonia, and dysmorphic features. Both a mild and a severe phenotype exist. The severe phenotype is homogeneous and is characterized by early infantile-onset epileptic encephalopathy and cardiomyopathy. The mild phenotype has a more variable clinical presentation. Diagnosis is based on the presence of an excess of D-2-hydroxyglutaric acid in the urine. Similarity: Belongs to the FAD-binding oxidoreductase/transferase type 4 family. Contains 1 FAD-binding PCMH-type domain.
  • Alternative names

    • D-2-hydroxyglutarate dehydrogenase
    • D-2-hydroxyglutarate dehydrogenase, mitochondrial
    • D2HGD
    • D2hgdh

Images

  • NADH Standard Curve
    NADH Standard Curve
  • Kinetic measurement of D2HGDH activity in various samples.
    Kinetic measurement of D2HGDH activity in various samples.
  • ) D2HGDH specific activity was calculated in lysates prepared from Rat Liver (5 µg) and Rat Kidney (4 µg)
    ) D2HGDH specific activity was calculated in lysates prepared from Rat Liver (5 µg) and Rat Kidney (4 µg)

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab239706? Please let us know so that we can cite the reference in this datasheet.

    ab239706 has not yet been referenced specifically in any publications.

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