Overview

  • Product name

  • Description

    Rabbit polyclonal to D4 GDI
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human D4 GDI aa 50-100. The exact sequence is proprietary.
    Database link: P52566

  • Positive control

    • WB: Jurkat whole cell lysate (ab7899). IP: Jurkat whole cell lysate (ab7899). IHC-P: Human stomach carcinoma inflammatory cell infiltrate.

Properties

Applications

Our Abpromise guarantee covers the use of ab226350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 22 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them (PubMed:8356058, PubMed:7512369). Regulates reorganization of the actin cytoskeleton mediated by Rho family members (PubMed:8262133).
  • Tissue specificity

    Detected in bone marrow, thymus and spleen.
  • Sequence similarities

    Belongs to the Rho GDI family.
  • Cellular localization

    Cytoplasm, cytosol.
  • Information by UniProt
  • Database links

  • Alternative names

    • Arhgdib antibody
    • D4 antibody
    • D4 GDP dissociation inhibitor antibody
    • GDIA 2 antibody
    • GDIA2 antibody
    • GDID 4 antibody
    • GDID4 antibody
    • GDIR2_HUMAN antibody
    • GDP dissociation inhibitor D4 antibody
    • LY GDI antibody
    • Ly-GDI antibody
    • LYGDI antibody
    • MGC108926 antibody
    • RAP1GN1 antibody
    • Rho GDI 2 antibody
    • Rho GDI beta antibody
    • Rho GDI2 antibody
    • Rho GDP dissociation inhibitor (GDI) beta antibody
    • Rho GDP dissociation inhibitor 2 antibody
    • Rho GDP dissociation inhibitor beta antibody
    • Rho GDP-dissociation inhibitor 2 antibody
    • Rho-GDI beta antibody
    • RhoGDI2 antibody
    see all

Images

  • All lanes : Anti-D4 GDI antibody (ab226350) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 22 kDa


    Exposure time: 10 seconds


    Lysates prepared using NETN lysis buffer.

  • D4 GDI was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226350 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226350 at 0.4 µg/ml.

    Lane 1: ab226350 IP in Jurkat whole cell lysate.

    Lane 2: Control IgG IP in Jurkat whole cell lysate.

    Detection: Chemiluminescence with exposure time of 10 seconds.

  • Formalin-fixed, paraffin-embedded human stomach carcinoma inflammatory infiltrate stained for D4 GDI using ab226350 at 1/1000 dilution in immunohistochemical analysis.

    Detection: DAB staining.

References

ab226350 has not yet been referenced specifically in any publications.

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