Key features and details
- Rabbit polyclonal to D4 GDI
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-D4 GDI antibody
See all D4 GDI primary antibodies
DescriptionRabbit polyclonal to D4 GDI
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human D4 GDI aa 50-100. The exact sequence is proprietary.
Database link: P52566
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226350 was affinity purified using an epitope specific to D4 GDI immobilized on solid support.
Our Abpromise guarantee covers the use of ab226350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 22 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionRegulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them (PubMed:8356058, PubMed:7512369). Regulates reorganization of the actin cytoskeleton mediated by Rho family members (PubMed:8262133).
Tissue specificityDetected in bone marrow, thymus and spleen.
Sequence similaritiesBelongs to the Rho GDI family.
Cellular localizationCytoplasm, cytosol.
- Information by UniProt
- Arhgdib antibody
- D4 antibody
- D4 GDP dissociation inhibitor antibody
All lanes : Anti-D4 GDI antibody (ab226350) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 22 kDa
Exposure time: 10 seconds
Lysates prepared using NETN lysis buffer.
D4 GDI was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226350 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226350 at 0.4 µg/ml.
Lane 1: ab226350 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
Formalin-fixed, paraffin-embedded human stomach carcinoma inflammatory infiltrate stained for D4 GDI using ab226350 at 1/1000 dilution in immunohistochemical analysis.
Detection: DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226350 has been referenced in 1 publication.
- Liu W et al. RhoGDI2 positively regulates the Rho GTPases activation in response to the ß2 outside-in signaling in T cells adhesion and migration on ICAM-1. J Leukoc Biol 106:431-446 (2019). PubMed: 31075185