Key features and details
- Rabbit polyclonal to D4 GDI
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-D4 GDI antibody
See all D4 GDI primary antibodies
DescriptionRabbit polyclonal to D4 GDI
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
- WB: Jurkat, Raji and THP-1 whole cell lysates. ICC/IF: HepG2 cells.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab236593 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 22 kDa.|
|ICC/IF||1/50 - 1/200.|
FunctionRegulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them (PubMed:8356058, PubMed:7512369). Regulates reorganization of the actin cytoskeleton mediated by Rho family members (PubMed:8262133).
Tissue specificityDetected in bone marrow, thymus and spleen.
Sequence similaritiesBelongs to the Rho GDI family.
Cellular localizationCytoplasm, cytosol.
- Information by UniProt
- Arhgdib antibody
- D4 antibody
- D4 GDP dissociation inhibitor antibody
All lanes : Anti-D4 GDI antibody (ab236593) at 1/2000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : THP-1 (human monocytic leukemia cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 22 kDa
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for D4 GDI (green) using ab236593 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236593 has not yet been referenced specifically in any publications.