Overview

  • Product name

    Anti-D4 GDI antibody [EPR14211(B)]
    See all D4 GDI primary antibodies
  • Description

    Rabbit monoclonal [EPR14211(B)] to D4 GDI
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human D4 GDI aa 1-100. The exact sequence is proprietary.
    Database link: P52566

  • Positive control

    • Jurkat, U937, HL60 and Human bone marrow lysates; Human spleen tissue; HL60 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100.
IP 1/40 - 1/60.

Target

  • Function

    Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them (PubMed:8356058, PubMed:7512369). Regulates reorganization of the actin cytoskeleton mediated by Rho family members (PubMed:8262133).
  • Tissue specificity

    Detected in bone marrow, thymus and spleen.
  • Sequence similarities

    Belongs to the Rho GDI family.
  • Cellular localization

    Cytoplasm, cytosol.
  • Information by UniProt
  • Database links

  • Alternative names

    • Arhgdib antibody
    • D4 antibody
    • D4 GDP dissociation inhibitor antibody
    • GDIA 2 antibody
    • GDIA2 antibody
    • GDID 4 antibody
    • GDID4 antibody
    • GDIR2_HUMAN antibody
    • GDP dissociation inhibitor D4 antibody
    • LY GDI antibody
    • Ly-GDI antibody
    • LYGDI antibody
    • MGC108926 antibody
    • RAP1GN1 antibody
    • Rho GDI 2 antibody
    • Rho GDI beta antibody
    • Rho GDI2 antibody
    • Rho GDP dissociation inhibitor (GDI) beta antibody
    • Rho GDP dissociation inhibitor 2 antibody
    • Rho GDP dissociation inhibitor beta antibody
    • Rho GDP-dissociation inhibitor 2 antibody
    • Rho-GDI beta antibody
    • RhoGDI2 antibody
    see all

Images

  • Anti-D4 GDI antibody [EPR14211(B)] (ab181259) at 1/10000 dilution + Human bone marrow lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 23 kDa

  • Western blot analysis of immunoprecipitation pellet from Jurkat cell lysate (lane 1) or a negative control (lane 2) immunoprecipitated using ab181259 at 1/50 dilution.
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

     

  • Immunofluorescent analysis of HL60 cells (-20°Acetone-fixed) labeling D4 GDI with ab181259 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling D4 GDI with ab181259 at 1/2000 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

  • All lanes : Anti-D4 GDI antibody [EPR14211(B)] (ab181259) at 1/50000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : U937 cell lysate
    Lane 3 : HL60 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 23 kDa

References

ab181259 has not yet been referenced specifically in any publications.

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