Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14211(B)] to D4 GDI
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Product nameAnti-D4 GDI antibody [EPR14211(B)]
See all D4 GDI primary antibodies
DescriptionRabbit monoclonal [EPR14211(B)] to D4 GDI
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human D4 GDI aa 1-100. The exact sequence is proprietary.
Database link: P52566
- Jurkat, U937, HL60 and Human bone marrow lysates; Human spleen tissue; HL60 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).|
|IHC-P||1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||1/40 - 1/60.|
FunctionRegulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them (PubMed:8356058, PubMed:7512369). Regulates reorganization of the actin cytoskeleton mediated by Rho family members (PubMed:8262133).
Tissue specificityDetected in bone marrow, thymus and spleen.
Sequence similaritiesBelongs to the Rho GDI family.
Cellular localizationCytoplasm, cytosol.
- Information by UniProt
- Arhgdib antibody
- D4 antibody
- D4 GDP dissociation inhibitor antibody
Anti-D4 GDI antibody [EPR14211(B)] (ab181259) at 1/10000 dilution + Human bone marrow lysate at 10 µg
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 23 kDa
Western blot analysis of immunoprecipitation pellet from Jurkat cell lysate (lane 1) or a negative control (lane 2) immunoprecipitated using ab181259 at 1/50 dilution.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Immunofluorescent analysis of HL60 cells (-20°Acetone-fixed) labeling D4 GDI with ab181259 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling D4 GDI with ab181259 at 1/2000 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
All lanes : Anti-D4 GDI antibody [EPR14211(B)] (ab181259) at 1/50000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : U937 cell lysate
Lane 3 : HL60 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 23 kDa
ab181259 has not yet been referenced specifically in any publications.