Overview

  • Product name

    Anti-D6 antibody
  • Description

    Rabbit polyclonal to D6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human D6 (C terminal). The exact sequence is proprietary.
    Database link: O00590

  • Positive control

    • Human Lung respiratory epithelium; HepG2 cell extracts; COS7 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride

    PBS (without Mg2+, Ca2+)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab178503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Predicted molecular weight: 43 kDa.
ICC/IF 1/100 - 1/500.

Target

  • Relevance

    Chemokines and their receptor-mediated signal transduction are critical for the recruitment of effector immune cells to the inflammation site. Chemokine receptor D6 is expressed in a range of tissues and hemopoietic cells. The expression of this receptor in lymphatic endothelial cells and overexpression in vascular tumors suggested its function in chemokine-driven recirculation of leukocytes and possible chemokine effects on the development and growth of vascular tumors. This receptor appears to bind the majority of beta-chemokine family members.
  • Cellular localization

    Cell Membrane
  • Database links

  • Alternative names

    • ACKR2 antibody
    • Atypical chemokine receptor 2 antibody
    • C C chemokine receptor D6 antibody
    • CCBP2 antibody
    • CCR10 antibody
    • CCR9 antibody
    • Chemokine binding protein 2 antibody
    • Chemokine receptor CCR 10 antibody
    • Chemokine receptor CCR 9 antibody
    • Chemokine receptor D6 antibody
    • CMKBR9 antibody
    • D6 antibody
    • hD6 antibody
    see all

Images

  • All lanes : Anti-D6 antibody (ab178503) at 1/500 dilution

    Lane 1 : HepG2 cell extracts
    Lane 2 : HepG2 cell extracts with synthesized peptide

    Predicted band size: 43 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung respiratory epithelium tissue labeling D6 with ab178503 at 5 µg/ml.

  • Immunofluorescent analysis of COS7 cells labeling D6 with ab178503 at 1/100 dilution. The image on the right is treated with the synthesized peptide.

References

ab178503 has not yet been referenced specifically in any publications.

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