Key features and details
- Rabbit polyclonal to D6
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-D6 antibody
DescriptionRabbit polyclonal to D6
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide within Human D6 (C terminal). The exact sequence is proprietary.
Database link: O00590
- Human Lung respiratory epithelium; HepG2 cell extracts; COS7 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
PBS (without Mg2+, Ca2+)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab178503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/500 - 1/1000. Predicted molecular weight: 43 kDa.|
|ICC/IF||1/100 - 1/500.|
RelevanceChemokines and their receptor-mediated signal transduction are critical for the recruitment of effector immune cells to the inflammation site. Chemokine receptor D6 is expressed in a range of tissues and hemopoietic cells. The expression of this receptor in lymphatic endothelial cells and overexpression in vascular tumors suggested its function in chemokine-driven recirculation of leukocytes and possible chemokine effects on the development and growth of vascular tumors. This receptor appears to bind the majority of beta-chemokine family members.
Cellular localizationCell Membrane
- ACKR2 antibody
- Atypical chemokine receptor 2 antibody
- C C chemokine receptor D6 antibody
All lanes : Anti-D6 antibody (ab178503) at 1/500 dilution
Lane 1 : HepG2 cell extracts
Lane 2 : HepG2 cell extracts with synthesized peptide
Predicted band size: 43 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung respiratory epithelium tissue labeling D6 with ab178503 at 5 µg/ml.
Immunofluorescent analysis of COS7 cells labeling D6 with ab178503 at 1/100 dilution. The image on the right is treated with the synthesized peptide.
ab178503 has not yet been referenced specifically in any publications.