• Product name
  • Description
    Rabbit polyclonal to DAAM1
  • Host species
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 396-445 (GNTVQYWLLL DRIIQQIVIQ NDKGQDPDST PLENFNIKNV VRMLVNENEV) of human DAAM1 (NP_055807).

  • Positive control
    • 293T cell lysate.



Our Abpromise guarantee covers the use of ab84526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 123 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • Relevance
    DAAM1 (Dishevelled associated activator of morphogenesis 1) is a diaphanous-related formin which binds to dishevelled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex assembly. It is an essential component of noncanonical Wnt signaling. DAAM1 also communicates with Rho GTPases, CIP4 and Src in the regulation of the signalling pathways that co-ordinate the dynamics of the actin filament system. DAAM1 may be a coordinator of endocytosis and cytoskeletal remodeling.
  • Cellular localization
    Cytoplasmic. Note: Perinuclear.
  • Database links
  • Alternative names
    • DAAM 1 antibody
    • Dishevelled associated activator of morphogenesis 1 antibody
    • FLJ41657 antibody
    • KIAA0666 antibody


  • Anti-DAAM1 antibody (ab84526) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 123 kDa
    Observed band size: 144 kDa (why is the actual band size different from the predicted?)

    Gel concentration: 6-18%


ab84526 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your reply and sorry for the delay in mine.

I think the band at ˜150 kDa could well be the expected band. You could see this clearer by performing your experiment using the siRNA experiment you carried out.

However, I ca...

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Thank you for your reply and sharing your results with me.

It seems from the image that the BSA blocking has been much more successful. Although the band at ˜50 kDa has remained, there is now a clear band at around the expected 150 kDa. This i...

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Thank you for taking time to provide that extra information. It has made understanding what may be contributing to the problems observed much easier to ascertain.

From the results shared of the blot performed with the xxxxxx antibody it appear...

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Thank you for your reply and sorry for the delay in getting back to you.

xxxx is currently away from the office but I will try to help as best I can in this case. The information you have provided in regards to the experiment you have carried ...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable...

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