Overview

  • Product name
    DAB Substrate Kit
  • Product overview

    DAB substrate kit for IHC staining using peroxidase-based detection (ab64238). Ideal for autostainers as reagent is stable for up to 6 hours after mixing the two components.


    3,3'Diaminobenzidine (DAB) is a widely used chromogen for immunohistochemical staining. In the presence of peroxidase enzyme, DAB produces a brown precipitate that is insoluble in alcohol.


    This product is a two component form consisting of a liquid, refrigerator stable DAB Chromogen and DAB Substrate. 

  • Notes

    Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

  • Tested applications
    Suitable for: IHC-Pmore details

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Storage buffer
    Constituents: 100% Propylene glycol, 5% Hydrogen peroxide, DAB substrate buffer
  • Components 60 ml 125 ml
    50x DAB Chromogen 1 x 2ml 1 x 4ml
    DAB substrate 1 x 60ml 1 x 125ml
  • Research areas
  • Relevance
    3,3' Diaminobenzidine (DAB) is a widely used chromogen for immunohistochemical staining. In the presence of peroxidase enzyme, DAB produces a brown precipitate that is insoluble in alcohol.
  • Alternative names
    • 3 3' Diaminobenzidine

Applications

Our Abpromise guarantee covers the use of ab64238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.

Images

  • Immunohistochemical analysis of healthy and colitis mouse colon sections (untreated and treated with enoxaparin) labeling claudin-4 with ab15104 at 1/200. ab7090, anti-rabbit immunoglobulin G conjugated to horseradish peroxidase (HRP) at 1/300 was used as the secondary antibody. ab64238 at 1/50 was used to develop the histological signal.

    Antigen retrieval was performed by incubating the sections for 10 minutes at 97°C in 1 mM EDTA buffer, pH 8.0 or 10 mM citrate buffer, pH 6.0.

    Control, C; untreated colitis, DSS; oral enoxaparin, OE; intraperitoneal injection of enoxaparin, IPE.

  • Immuno-staining of formalin–fixed and paraffin-embedded liver sections with ab64238. Secontions were obtained from control (saline) and treated with CCl4+CT-N and CCl4+AD-N (left) and antibody control (right). 

  • Immunohistochemical analysis of mouse lung. Samples were fixed in 10% buffered formalin, paraffinized and sliced at 1.5 µm thick. Antigen retrieval was performed using ab64214 for the deparaffinized slices. Sections were blocked with 2% normal goat serum, PBS(-) and 0.1% Tween20. They were then incubated with the primary antibodies for 1 hour at 4°C and with secondary antibodies for 30 minutes at room temperature. The avidin-biotin-peroxidase complex method with peroxidase streptavidin and the DAB substrate kit ab64238 was performed.

    (A) A resected lung from a mouse sacrificed 28 days after SCL injection.

    (B) Haemotoxylin and Eosin staining showing the tumor composed of a central area with necrosis and a peripheral zone filled with SCLs.

    (C) Immunohistochemical staining for MMP-14 showing positive expression of MMP-14 in the peripheral zone of the tumor and negative in central zone.

    (D) Immunohistochemical staining weak staining of MMP-2 in the peripheral zone. 

References

This product has been referenced in:
  • Dan C  et al. HNF1B expression regulates ECI2 gene expression, potentially serving a role in prostate cancer progression. Oncol Lett 17:1094-1100 (2019). Read more (PubMed: 30655870) »
  • Zhao L  et al. Label-free microfluidic chip for the identification of mesothelial cell clusters in pleural effusion. Oncol Lett 17:4532-4544 (2019). Read more (PubMed: 30944642) »
See all 44 Publications for this product

Customer reviews and Q&As

Filter by Ratings

1-3 of 3 Abreviews

Abreviews
This DAB substrate kit (ab94665) is easy to use, and the quality is good.


Liyong Zhang

Abcam user community

Verified customer

Submitted Aug 15 2017

Abreview for DAB substrate kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews
This kit is streamlined to use at ease. The results is fantastic.


Liyong Zhang

Abcam user community

Verified customer

Submitted Aug 15 2017

IHC-P Mouse pancreas

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Ki67 IHC staining on mouse pancreas, blocking with 10% BSA, 1 hr ab incubation RT

Abcam user community

Verified customer

Submitted Aug 16 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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