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Please see below an e-mail, we need to do complete Rabies IHC test... i.e all materials from Antibody diluent to retrival to everything..
Please advice me ASAP.. Also the customer needs specific protocol, as she is very new to IHC.
Asked on Jul 31 2012
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Here is the list with the requested items. However, please be aware that this is not a technical inquiry as all item could be found by using the search application on our website under the "reagents" category. Please let us know if you need help on navigating our website so you can help your customers faster than relying on us.
Regarding the Rabies antibody, I am afraid we do not have what your customer requests: All we have are mouse monoclonal antibodies against this virus, but none produced in goat. Please let me know if this expectable for her, so I can suggest the right primary antibody with the matching biotinylated secondary antibody.
https://www.abcam.com/10x-Citrate-Buffer-pH-60-ab64214.html (or use the following: https://www.abcam.com/10x-Citrate-Buffer-pH-60-ab64214.html).
https://www.abcam.com/10x-EDTA-Buffer-pH-80-ab64216.html (or use the following: https://www.abcam.com/10x-EDTA-Buffer-pH-80-ab64216.html).
https://www.abcam.com/DAB-Substrate-Kit-ab64238.html (or use the following: https://www.abcam.com/DAB-Substrate-Kit-ab64238.html).
https://www.abcam.com/Streptavidin-HRP-ready-to-use-ab64269.html (or use the following: https://www.abcam.com/Streptavidin-HRP-ready-to-use-ab64269.html).
https://www.abcam.com/Hydrogen-Peroxide-Blocking-Reagent-ab94666.html (or use the following: https://www.abcam.com/Hydrogen-Peroxide-Blocking-Reagent-ab94666.html).
https://www.abcam.com/Protein-Block-ab128988.html (or use the following: https://www.abcam.com/Protein-Block-ab128988.html).
https://www.abcam.com/Mayers-Hematoxylin-ab128990.html (or use the following: https://www.abcam.com/Mayers-Hematoxylin-ab128990.html).
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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Answered on Jul 31 2012