Overview

  • Product name
  • Description
    Goat polyclonal to Dab1
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Fusion protein, corresponding to amino acids 1-179 of Mouse Dab1.

  • Positive control
    • WB: 293 cells transfected with Dab1–HA tag. IP: Mouse p14 brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab16674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 67 kDa).
IP Use a concentration of 5 - 10 µg/ml.

Target

  • Function
    Adapter molecule functioning in neural development. May regulate SIAH1 activity.
  • Sequence similarities
    Contains 1 PID domain.
  • Domain
    The PID domain specifically binds to the Asn-Pro-Xaa-Tyr(P) motif found in many tyrosine-phosphorylated proteins.
  • Post-translational
    modifications
    Phosphorylated on Tyr-198 and Tyr-220 upon reelin induction in embryonic neurons (By similarity). Also phosphorylated on Ser-524 independently of reelin signaling.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dab 1 antibody
    • Dab reelin signal transducer 1 antibody
    • Dab, reelin signal transducer, homolog 1 (Drosophila) antibody
    • Dab1 antibody
    • DAB1_HUMAN antibody
    • Disabled homolog 1 antibody
    • Disabled homolog 1 Drosophila antibody
    • Scm antibody
    • Scr antibody
    • Scrambler antibody
    • Yot antibody
    • Yotari antibody
    see all

Images

  • ab16674 detecting mouse Dab1 in brain extracts by Western blot.

    Lane 1: ab16674 (N terminal).

    Lane 2: ab16675 (C terminal).

    ab16674 detecting mouse Dab1 in brain extracts by Western blot. Lane 1: ab16674 (N terminal). Lane 2: ab16675 (C terminal).

References

ab16674 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (THP-1)
Total protein in input
50 µg
Specification
THP-1
Treatment
200 nM PMA for 24 hours. Cells in sample 2 were treated with 300 uM hydrogen peroxide for 20 mins before being lysed.
Immuno-precipitation step
Protein A/G

Abcam user community

Verified customer

Submitted Aug 07 2012

Answer

Thank you for confirming those details.

As requested, I have generated the testing discount code for you as below:

Discount code: xxxxx

Expiration date: 5th November 2012

Please orderab111684 as normalandquote this discountcode in the "Additional comments" section when submitting your resultswith IP in the Abreview for ab111684.Once the Abreview has been submitted the code will become activated and you can quote the code when placing the order for your free product. This code expires on the 5th November 2012. More information on the testing discount scheme can be found form the following link:

https://www.abcam.com/collaboratordiscount

Please do not worry about the email you sent to us. I have marked this as completed. Thank you for flagging this up to me.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

Until then, I wish you all the best with yourexperiments.

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Question
Answer

Thank you for contacting us yesterday in regards to antibodies to be used in immunoprecipitation of Dab1 from mouse and human samples.

Unfortunately, as discussed over the phone, we do not currently have any antibodies in our catalogue which have been used in immunoprecipitation with both mouse and human samples. The two antibodies we have where they have been shown to work in immunoprecipitation,ab16674 and ab16675, haveonly as yet been tested with mouse samples. The homology of the immunogens used are however very similar and we would therefore expect these antibodies to be able to detect the human protein as well (please refer to the sequence alignment attached).Especially with ab16674 where the homology is 100% (immunogen composed of residues 1-179 of mouse Dab1).

However, as we have not tested these antibodies with human samples we would not be able to guarantee that they would work.If you would like to purchase ab16674or ab16675 and use it with yourhuman samples, we can offer you a testing discount. This offer means that if you purchaseeither of these antibodiesas normal, test the antibody with yourhumansamples and let us know of the results through an Abreview (no matter whether positive or negative) you would be eligible for a free primary antibody of your choice from our catalogue (or the equivalent price of the antibody off any product). More information on this offer can be found here:

www.abcam.com/collaborationdiscount

Please not that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period. If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab16674or ab16675.

Alternatively, I would be able to offer the same testing discount if you wanted to try the ab111684 instead. This antibody has been shown to detect both the mouse and human Dab1 protein but has as yet not been used in immunoprecipitation. We would therefore not be able to guarantee that it would work in this application.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Adult retina)
Specification
Adult retina
Fixative
Formaldehyde
Blocking step
Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Jun 14 2006

Answer

Thank you for your enquiry. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will award you 50 points with the Abcam Loyalty Scheme which can be redeemed on a number of rewards.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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