Overview

  • Product name

    Anti-Dab1 (phospho Y232) antibody
    See all Dab1 primary antibodies
  • Description

    Rabbit polyclonal to Dab1 (phospho Y232)
  • Host species

    Rabbit
  • Specificity

    This antibody detects endogenous levels of Dab1 only when phosphorylated at tyrosine 232.
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesized phosphopeptide derived from human Dab1 around the phosphorylation site of tyrosine 232 (G-V-YP-D-V).

  • Positive control

    • Extracts from LOVO cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab78200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
ELISA 1/20000.

Target

  • Function

    Adapter molecule functioning in neural development. May regulate SIAH1 activity.
  • Sequence similarities

    Contains 1 PID domain.
  • Domain

    The PID domain specifically binds to the Asn-Pro-Xaa-Tyr(P) motif found in many tyrosine-phosphorylated proteins.
  • Post-translational
    modifications

    Phosphorylated on Tyr-198 and Tyr-220 upon reelin induction in embryonic neurons (By similarity). Also phosphorylated on Ser-524 independently of reelin signaling.
  • Information by UniProt
  • Database links

  • Alternative names

    • Dab 1 antibody
    • Dab reelin signal transducer 1 antibody
    • Dab, reelin signal transducer, homolog 1 (Drosophila) antibody
    • Dab1 antibody
    • DAB1_HUMAN antibody
    • Disabled homolog 1 antibody
    • Disabled homolog 1 Drosophila antibody
    • Scm antibody
    • Scr antibody
    • Scrambler antibody
    • Yot antibody
    • Yotari antibody
    see all

Images

  • All lanes : Anti-Dab1 (phospho Y232) antibody (ab78200) at 1/500 dilution

    Lane 1 : Extracts from LOVO cells
    Lane 2 : Extracts from LOVO cells plus 5µg immunizing peptide

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 67 kDa
    Observed band size: 67 kDa

References

This product has been referenced in:

  • Rogers JT  et al. Reelin supplementation enhances cognitive ability, synaptic plasticity, and dendritic spine density. Learn Mem 18:558-64 (2011). Read more (PubMed: 21852430) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (embryo - kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer, pH 6.0
Permeabilization
No
Specification
embryo - kidney
Blocking step
No blocking step used for 20 minute(s) · Temperature: RT°C
Fixative
Paraformaldehyde

Miss. Anita Muić

Verified customer

Submitted Oct 18 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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