Recombinant
RabMAb

Anti-Dab1 (phospho Y232) antibody [EPR2247(2)] (ab126728)

Overview

  • Product name
    Anti-Dab1 (phospho Y232) antibody [EPR2247(2)]
    See all Dab1 primary antibodies
  • Description
    Rabbit monoclonal [EPR2247(2)] to Dab1 (phospho Y232)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Phospho specific peptide corresponding to residues surrounding Tyrosine 232 of Human Dab1.

  • Positive control
    • SH-SY5Y cells and lysates treated with pervanadate
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab126728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 80 kDa (predicted molecular weight: 64 kDa).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function
      Adapter molecule functioning in neural development. May regulate SIAH1 activity.
    • Sequence similarities
      Contains 1 PID domain.
    • Domain
      The PID domain specifically binds to the Asn-Pro-Xaa-Tyr(P) motif found in many tyrosine-phosphorylated proteins.
    • Post-translational
      modifications
      Phosphorylated on Tyr-198 and Tyr-220 upon reelin induction in embryonic neurons (By similarity). Also phosphorylated on Ser-524 independently of reelin signaling.
    • Information by UniProt
    • Database links
    • Alternative names
      • Dab 1 antibody
      • Dab reelin signal transducer 1 antibody
      • Dab, reelin signal transducer, homolog 1 (Drosophila) antibody
      • Dab1 antibody
      • DAB1_HUMAN antibody
      • Disabled homolog 1 antibody
      • Disabled homolog 1 Drosophila antibody
      • Scm antibody
      • Scr antibody
      • Scrambler antibody
      • Yot antibody
      • Yotari antibody
      see all

    Images

    • All lanes : Anti-Dab1 (phospho Y232) antibody [EPR2247(2)] (ab126728) at 1/10000 dilution

      Lane 1 : untreated SH-SY5Y cell lysate
      Lane 2 : pervanadate treated SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 64 kDa
      Observed band size: 80 kDa (why is the actual band size different from the predicted?)

    • ab126728, at a dilution of 1/10 staining Dab1 (phospho Y232) in permeabilized pervanadate-treated (red) or untreated (negative) (green) SH-SY5Y cells by Flow Cytometry. Using ab126728 preincubated with phospho-Dab1 Phospho (pY232) peptide (blue) or non-phospho-Dab1 Phospho (pY232) peptide (orange).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Brai E  et al. Notch1 Regulates Hippocampal Plasticity Through Interaction with the Reelin Pathway, Glutamatergic Transmission and CREB Signaling. Front Cell Neurosci 9:447 (2015). WB ; Mouse . Read more (PubMed: 26635527) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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