• Product name
  • Description
    Rabbit polyclonal to DAB2
  • Host species
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Zebrafish, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 750 to the C-terminus of Human DAB2.

    Read Abcam's proprietary immunogen policy (Peptide available as ab33440.)

  • Positive control
    • This antibody gave a positive signal in Human Testis Tissue Lysate. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MCF-7



Our Abpromise guarantee covers the use of ab33441 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 102 kDa (predicted molecular weight: 83, 58.8 kDa).


  • Relevance
    DAB2 is a component of the CSF1 signal transduction pathway. DAB2 mRNA is expressed in normal ovarian epithelial cells but is down regulated or absent from ovarian carcinoma cell lines. The down regulation of DAB2 may play an important role in ovarian carcinogenesis. This gene was initially named DOC2 (for Differentially expressed in Ovarian Cancer) and is distinct from the DOC2A and DOC2B genes (for double C2 like domains, alpha and beta).
  • Cellular localization
    Cytoplasmic vesicle; clathrin coated vesicle; clathrin coated vesicle membrane. Cell membrane; coated pit; clathrin coated pit. Note: Colocalizes with large insert containing isoforms of MYO6 at clathrin coated pits/vesicles.
  • Database links
  • Alternative names
    • DAB 2 antibody
    • Dab, mitogen-responsive phosphoprotein, homolog 2 (Drosophila) antibody
    • DAB2 antibody
    • Differentially expressed in ovarian cancer 2 antibody
    • Differentially expressed protein 2 antibody
    • Disabled (Drosophila) homolog 2 (mitogen responsive phosphoprotein) antibody
    • Disabled homolog 2 antibody
    • Disabled homolog 2 mitogen responsive phosphoprotein (Drosophila) antibody
    • Disabled homolog 2 mitogen responsive phosphoprotein antibody
    • Disabled, Drosophila, homolog of, 2 antibody
    • DOC 2 antibody
    • DOC2 antibody
    • FLJ26626 antibody
    • Mitogen responsive phosphoprotein antibody
    see all


  • Anti-DAB2 antibody (ab33441) at 2 µg/ml + Human testis tissue lysate - total protein (ab30257) at 10 µg

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 83, 58.8 kDa
    Observed band size: 102 kDa
    why is the actual band size different from the predicted?

    This antibody was raised against an immunogen that is predicted to cross react with both isoforms 1 and 2 of Human DAB2 .  We believe that the band observed is isoform 1 due to its higher molecular weight of 102 kDa.  While this differs to its predicted molecular weight of 83 kDa (Swiss-Prot data) this migration has been observed in the literature  and may be due to modifications at potential phosphorylation sites. (Am J Pathol. 2006 Jul;169(1):258-67.  PMID: 16816378).

  • ICC/IF image of ab33441 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab33441 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
  • Cook A  et al. Application of the SSB biosensor to study in vitro transcription. Biochem Biophys Res Commun 496:820-825 (2018). RT-PCR . Read more (PubMed: 29378185) »
  • Ma S  et al. X-ray irradiation induced Disabled-2 gene promoter de-methylation enhances radiosensitivity of non-small-cell lung carcinoma cells. J Exp Clin Cancer Res 37:315 (2018). Read more (PubMed: 30547821) »
See all 3 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (placenta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C

Mr. Antibody Solutions

Verified customer

Submitted May 25 2009


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