• Product name
  • Description
    Rabbit polyclonal to DAB2IP
  • Host species
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residue 850 and 900 of human DAB2IP (CAQ10385.1).

  • Positive control
    • Whole cell lysate from HeLa cells.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab87811 was affinity purified using an epitope specific to DAB2IP immobilized on solid support.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab87811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 132 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P Use a concentration of 1 µg/ml.


  • Relevance
    DAB2IP is a Ras GTPase-activating protein (GAP) that acts as a tumor suppressor gene and is inactivated by methylation in prostate and breast cancers. It also interacts with MAP3K5 to disrupt the association of MAP3K5 with the inhibitory 14-3-3 complex.
  • Cellular localization
  • Database links
  • Alternative names
    • AF9Q34 antibody
    • AIP1 antibody
    • ASK interacting protein antibody
    • ASK interacting protein 1 antibody
    • ASK1 interacting protein 1 antibody
    • DAB2 interacting protein antibody
    • DAB2 interaction protein antibody
    • DAB2P antibody
    • DIP1/2 antibody
    • Disabled homolog 2 interacting protein antibody
    • DOC 2/DAB2 interactive protein antibody
    • FLJ39072 antibody
    • KIAA1743 antibody
    • nGAP like protein antibody
    see all


  • All lanes : Anti-DAB2IP antibody (ab87811) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Predicted band size: 132 kDa
    Observed band size: 132 kDa

    Exposure time: 3 minutes
  • Detection of DAB2IP in immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab87811 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot.
    Detection: Chemiluminescence with an exposure time of 3 seconds.
  • IHC image of ab87811 staining in normal human breast formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab87811, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


This product has been referenced in:
  • Li S  et al. BMAL1 facilitates trophoblast migration and invasion via SP1-DNMT1/DAB2IP pathway in recurrent spontaneous abortion. Oncotarget 8:89451-89464 (2017). WB ; Human . Read more (PubMed: 29163762) »
  • Zheng L  et al. Low expression of DAB2IP predicts an unfavorable prognosis in human bladder carcinoma. Onco Targets Ther 10:5719-5726 (2017). Read more (PubMed: 29238204) »

See all 5 Publications for this product

Customer reviews and Q&As

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry the anti-DAB2IP antibody ab87811 did not perform as stated on the datasheet and for th...

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Thank you for confirming these details. The details you have kindly provided will provide us with vital information for our monitoring of product quality. Although it is not necessary to add further loading buffer to the lysates you purchased from us, ...

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