Overview

  • Product name
  • Description
    Rabbit polyclonal to DACH1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Horse, Chicken, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human DACH1 aa 720-740. The exact sequence is proprietary. (CAH73005.1).
    Sequence:

    SLRVLNDSLTPEIEADRSGGR


    Database link: Q9UI36

  • Positive control
    • 293T whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 99% Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab176718 was affinity purified using an epitope specific to DACH1 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab176718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 79 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator of SIX1, SIX6 and probably SIX5. Corepression of precursor cell proliferation in myoblasts by SIX1 is switched to coactivation through recruitment of EYA3 to the SIX1-DACH1 complex. Transcriptional activation seems also to involve association of CREBBP. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter (By similarity). Inhibits TGF-beta signaling through interaction with SMAD4 and NCOR1. Binds to chromatin DNA via its DACHbox-N domain.
  • Tissue specificity
    Widely expressed. Isoform 2 is found in brain, heart, kidney, liver, leukocytes and spleen. Isoform 3 is found in liver and heart. Isoform 4 is found in spleen.
  • Sequence similarities
    Belongs to the DACH/dachshund family.
  • Domain
    The DACHbox-N/DD1 domain forms a structure containing a DNA binding motif similar to that of the forkhead/winged helix domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DACH antibody
    • Dach1 antibody
    • DACH1_HUMAN antibody
    • Dachshund family transcription factor 1 antibody
    • dachshund homolog 1 (Drosophila) antibody
    • Dachshund homolog 1 antibody
    • Dachsund homolog 1 antibody
    • FLJ10138 antibody
    • OTTHUMP00000018494 antibody
    see all

Images

  • All lanes : Anti-DACH1 antibody (ab176718) at 0.04 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : Jurkat whole cell lysate at 50 µg
    Lane 4 : HeLa whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 79 kDa


    Exposure time: 30 seconds
  • Detection of DACH1 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab176718 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.

    Detection: Chemiluminescence with an exposure time of 3 seconds.

References

This product has been referenced in:
  • Kim SH  et al. Forkhead Box Q1 Is a Novel Target of Breast Cancer Stem Cell Inhibition by Diallyl Trisulfide. J Biol Chem 291:13495-508 (2016). Read more (PubMed: 27129776) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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