Key features and details
- Rabbit polyclonal to DACH1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DACH1 antibody
See all DACH1 primary antibodies
DescriptionRabbit polyclonal to DACH1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rabbit, Horse, Chicken, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
- Jurkat whole cell lysate (ab7899) can be used as a positive control in WB. 293T whole cell lysates.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab176718 was affinity purified using an epitope specific to DACH1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab176718 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 79 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionTranscription factor that is involved in regulation of organogenesis. Seems to be a regulator of SIX1, SIX6 and probably SIX5. Corepression of precursor cell proliferation in myoblasts by SIX1 is switched to coactivation through recruitment of EYA3 to the SIX1-DACH1 complex. Transcriptional activation seems also to involve association of CREBBP. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter (By similarity). Inhibits TGF-beta signaling through interaction with SMAD4 and NCOR1. Binds to chromatin DNA via its DACHbox-N domain.
Tissue specificityWidely expressed. Isoform 2 is found in brain, heart, kidney, liver, leukocytes and spleen. Isoform 3 is found in liver and heart. Isoform 4 is found in spleen.
Sequence similaritiesBelongs to the DACH/dachshund family.
DomainThe DACHbox-N/DD1 domain forms a structure containing a DNA binding motif similar to that of the forkhead/winged helix domain.
- Information by UniProt
- DACH antibody
- Dach1 antibody
- DACH1_HUMAN antibody
All lanes : Anti-DACH1 antibody (ab176718) at 0.04 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : Jurkat whole cell lysate at 50 µg
Lane 4 : HeLa whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 79 kDa
Exposure time: 30 seconds
Detection of DACH1 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab176718 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
Detection: Chemiluminescence with an exposure time of 3 seconds.
ab176718 has been referenced in 2 publications.
- Cheng Q et al. SIX1 and DACH1 influence the proliferation and apoptosis of hepatocellular carcinoma through regulating p53. Cancer Biol Ther 19:381-390 (2018). PubMed: 29333942
- Kim SH et al. Forkhead Box Q1 Is a Novel Target of Breast Cancer Stem Cell Inhibition by Diallyl Trisulfide. J Biol Chem 291:13495-508 (2016). PubMed: 27129776