Overview

  • Product name
    Anti-Dact1 / Dapper homolog 1 antibody
    See all Dact1 / Dapper homolog 1 primary antibodies
  • Description
    Rabbit polyclonal to Dact1 / Dapper homolog 1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IP, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fusion protein, corresponding to amino acids 280-516 of Human Dact1/ Dapper homolog 1

Properties

Applications

Our Abpromise guarantee covers the use of ab51260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/200.
IP 1/100 - 1/200.
WB 1/1000 - 1/5000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Positively regulates DVL2-mediated signaling pathways during development. Binds to DVL2 and impedes the degradation of CTNNB1/beta-catenin, thereby enhancing the transcriptional activation of target genes of the Wnt signaling pathway.
  • Sequence similarities
    Belongs to the dapper family.
  • Domain
    The C-terminal PDZ-binding motif mediates interaction with the PDZ domains of DSH (Dishevelled) family proteins.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dact antibody
    • dact1 antibody
    • DACT1_HUMAN antibody
    • Dapper antagonist of beta catenin homolog 1 antibody
    • Dapper antagonist of catenin 1 antibody
    • Dapper antibody
    • Dapper homolog 1 antibody
    • DAPPER1 antibody
    • Dpr antibody
    • Dpr1 antibody
    • Frd antibody
    • Frodo antibody
    • hDPR1 antibody
    • Hepatocellular carcinoma novel gene 3 protein antibody
    • Heptacellular carcinoma novel gene 3 antibody
    • HNG3 antibody
    • MTNG3 antibody
    • Thyex3 antibody
    see all

Images

  • All lanes : Anti-Dact1 / Dapper homolog 1 antibody (ab51260) at 1/1000 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : Molecular weight marker
    Lane 3 : HEK293 transfected with Myc-Dact1 cell lysate

    Predicted band size: 90 kDa
    Observed band size: 90 kDa



    Additional bands seen are thought to be degraded products of Dact1
  • HeLa cells stained with ab51260 Dact1/Dapper homolog 1 antibody (red) at a dilution of 1/200.
  • .... cell extracts immunoprecipitated with ab51260 at 1/200 dilution (lane 2) or a negative control antibody (lane 1). Immunoprecipitates were blotted with ab51260 at 1/1000 dilution.
  • IHC image of ab51260 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51260, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:
  • Tuo H  et al. MiR-324-3p promotes tumor growth through targeting DACT1 and activation of Wnt/ß-catenin pathway in hepatocellular carcinoma. Oncotarget 8:65687-65698 (2017). Read more (PubMed: 29029464) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Exocrine pancreas)
Permeabilization
No
Specification
Exocrine pancreas
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Acetone

Abcam user community

Verified customer

Submitted Jul 14 2017

Answer

Thank you for contacting us. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Stem)
Specification
Stem
Fixative
Paraformaldehyde
Permeabilization
Yes - Ethanol
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Mar 12 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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