Anti-DAD1 antibody (ab23836)

Reviews (1)Q&A (1)References (2)

Overview

  • Product name
    Anti-DAD1 antibody
    See all DAD1 primary antibodies
  • Description
    Rabbit polyclonal to DAD1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to a 14 amino acid sequence within the C terminal region of DAD 1 (Human).

  • Positive control
    • HepG2 cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    Purified by affinity chromatography.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab23836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 12 kDa).
ICC Use at an assay dependent dilution.

Target

  • Function
    Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss of the DAD1 protein triggers apoptosis.
  • Sequence similarities
    Belongs to the DAD/OST2 family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • DAD 1 antibody
      • DAD-1 antibody
      • dad1 antibody
      • DAD1_HUMAN antibody
      • Defender against cell death 1 antibody
      • Dolichyl diphosphooligosaccharide protein glycosyltransferase subunit DAD1 antibody
      • Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 antibody
      • Oligosaccharyl transferase subunit DAD1 antibody
      • OST 2 antibody
      • OST2 antibody
      see all

    Images

    • Western blot - Anti-DAD1 antibody (ab23836)
      Western blot - Anti-DAD1 antibody (ab23836)
      Lane 1 : Anti-DAD1 antibody (ab23836) at 0.5 µg/ml
      Lane 2 : Anti-DAD1 antibody (ab23836) at 1 µg/ml
      Lane 3 : Anti-DAD1 antibody (ab23836) at 2 µg/ml

      All lanes : HepG2 whole cell lysate

      Predicted band size: 12 kDa
      Observed band size: 22 kDa
      why is the actual band size different from the predicted?

    • Immunocytochemistry - Anti-DAD1 antibody (ab23836)
      Immunocytochemistry - Anti-DAD1 antibody (ab23836)
      ab23836 staining DAD1 in HepG2 cells by Immunocytochemistry.

    References

    This product has been referenced in:
    • Ding Y  et al. The endoplasmic reticulum-based acetyltransferases, ATase1 and ATase2, associate with the oligosaccharyltransferase to acetylate correctly folded polypeptides. J Biol Chem 289:32044-55 (2014). WB . Read more (PubMed: 25301944) »
    • Murooka TT  et al. CCL5 promotes proliferation of MCF-7 cells through mTOR-dependent mRNA translation. Biochem Biophys Res Commun 387:381-6 (2009). WB ; Human . Read more (PubMed: 19607806) »
    See all 2 Publications for this product

    Publishing research using ab23836? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-DAD1 antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (skin)
    Loading amount
    15 µg
    Specification
    skin
    Gel Running Conditions
    Non-reduced Denaturing (15% gel)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 24 2009

    Question

    I am thinking of purchasing DAD1 antibody to use against Xenopus Laevis. The primary accession number is P46967, and the fasta format follows. >sp|P46967|DAD1_XENLA Defender against cell death 1 (DAD-1) - Xenopus laevis (African clawed frog). MSVSVFSVVSRFLDEYVSSTPQRLKLLDAYLLYILLTGALQFLYCLLVGTFPFNSFLSGFISSVGSFILAVCLRIQINPQNKSDFQGISPERAFADFLFANTILHLVVVNFIG I was wondering if you could give me an idea of the chance that your anti body will detect this version of the protein? Thank you

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    Answer

    Thank you for your enquiry. I contacted the antibody originator regarding ab23836. Unfortunately the amino acid sequence of the immunogen is proprietary. The only information I have is that the sequence was selected from the last half of the protein, but not the very C-terminus. The amino acid sequence of human DAD1 is in the public domain (GenBank accession no. AAH09798). However, without access to the immunogen sequence I am not in a position to comment on the homology between the relevant sequence in human versus xenopus. I am sorry that I could not assist further. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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