Anti-DAD1 antibody (ab23836)
Key features and details
- Rabbit polyclonal to DAD1
- Suitable for: WB, ICC
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DAD1 antibody -
Description
Rabbit polyclonal to DAD1 -
Host species
Rabbit -
Tested Applications & Species
Application Species ICC HumanWB Human -
Immunogen
Synthetic peptide corresponding to a 14 amino acid sequence within the C terminal region of DAD 1 (Human).
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Positive control
- HepG2 cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified by affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab23836 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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ICC |
Human
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WB |
Human
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Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 12 kDa).
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ICC |
Use at an assay dependent dilution.
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Notes |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 12 kDa). |
ICC
Use at an assay dependent dilution. |
Target
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Function
Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss of the DAD1 protein triggers apoptosis. -
Sequence similarities
Belongs to the DAD/OST2 family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 1603 Human
- GenBank: NP_001335.1 Human
- Omim: 600243 Human
- SwissProt: P61803 Human
- Unigene: 82890 Human
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Alternative names
- DAD 1 antibody
- DAD-1 antibody
- dad1 antibody
see all
Images
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Lane 1 : Anti-DAD1 antibody (ab23836) at 0.5 µg/ml
Lane 2 : Anti-DAD1 antibody (ab23836) at 1 µg/ml
Lane 3 : Anti-DAD1 antibody (ab23836) at 2 µg/ml
All lanes : HepG2 whole cell lysate
Predicted band size: 12 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted? -
ab23836 staining DAD1 in HepG2 cells by Immunocytochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab23836 has been referenced in 2 publications.
- Ding Y et al. The endoplasmic reticulum-based acetyltransferases, ATase1 and ATase2, associate with the oligosaccharyltransferase to acetylate correctly folded polypeptides. J Biol Chem 289:32044-55 (2014). WB . PubMed: 25301944
- Murooka TT et al. CCL5 promotes proliferation of MCF-7 cells through mTOR-dependent mRNA translation. Biochem Biophys Res Commun 387:381-6 (2009). WB ; Human . PubMed: 19607806