Product nameAnti-DAD1 antibody
See all DAD1 primary antibodies
DescriptionRabbit polyclonal to DAD1
Tested applicationsSuitable for: WB, ICCmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to a 14 amino acid sequence within the C terminal region of DAD 1 (Human).
- HepG2 cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityProtein A purified
Purification notesPurified by affinity chromatography.
Our Abpromise guarantee covers the use of ab23836 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 12 kDa).|
|ICC||Use at an assay dependent dilution.|
FunctionComponent of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss of the DAD1 protein triggers apoptosis.
Sequence similaritiesBelongs to the DAD/OST2 family.
- Information by UniProt
- DAD 1 antibody
- DAD-1 antibody
- dad1 antibody
Lane 1 : Anti-DAD1 antibody (ab23836) at 0.5 µg/ml
Lane 2 : Anti-DAD1 antibody (ab23836) at 1 µg/ml
Lane 3 : Anti-DAD1 antibody (ab23836) at 2 µg/ml
All lanes : HepG2 whole cell lysate
Predicted band size: 12 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
ab23836 staining DAD1 in HepG2 cells by Immunocytochemistry.
This product has been referenced in:
- Ding Y et al. The endoplasmic reticulum-based acetyltransferases, ATase1 and ATase2, associate with the oligosaccharyltransferase to acetylate correctly folded polypeptides. J Biol Chem 289:32044-55 (2014). WB . Read more (PubMed: 25301944) »
- Murooka TT et al. CCL5 promotes proliferation of MCF-7 cells through mTOR-dependent mRNA translation. Biochem Biophys Res Commun 387:381-6 (2009). WB ; Human . Read more (PubMed: 19607806) »