DAF-2 DA, Fluorescent nitric oxide probe (ab145283)

Overview

  • Product name

    DAF-2 DA, Fluorescent nitric oxide probe
  • Description

    Cell-permeable fluorescent nitric oxide probe.
  • Alternative names

    • 4,5-Diaminofluorescein diacetate
    • 5,6-Diaminofluorescein diacetate
    • DAF-2 diacetate
  • Biological description

    Cell-permeable fluorescent nitric oxide (NO) indicator. Deacetylated by intracellular esterases to DAF-2 (ab145269). DAF-2 reacts with NO to yield the highly fluorescent triazolofluorescein (DAF-2T) (ab145473).

  • Purity

    > 98%
  • CAS Number

    205391-02-2
  • Chemical structure

    Chemical Structure

Properties

  • Excitation

    491nm
  • Emission

    513nm
  • Chemical name

    4,5-Diaminofluorescein diacetate
  • Molecular weight

    446.41
  • Molecular formula

    C24H18N2O7
  • Storage instructions

    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in DMSO
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • Source

    Synthetic

References

This product has been referenced in:

  • Zhou X & He P Improved measurements of intracellular nitric oxide in intact microvessels using 4,5-diaminofluorescein diacetate. Am J Physiol Heart Circ Physiol 301:H108-14 (2011). Read more (PubMed: 21536843) »
  • Takeno S  et al. Increased nitric oxide production in nasal epithelial cells from allergic patients--RT-PCR analysis and direct imaging by a fluorescence indicator: DAF-2 DA. Clin Exp Allergy 31:881-8 (2001). Read more (PubMed: 11422152) »
  • Kojima H  et al. Direct evidence of NO production in rat hippocampus and cortex using a new fluorescent indicator: DAF-2 DA. Neuroreport 9:3345-8 (1998). Read more (PubMed: 9855277) »

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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