DAF-2, Fluorescent nitric oxide probe (ab145269)

Overview

  • Product name

    DAF-2, Fluorescent nitric oxide probe
  • Description

    Highly sensitive, specific fluorescent nitric oxide probe.
  • Alternative names

    • 4,5-Diaminofluorescein
  • Biological description

    Highly sensitive, specific fluorescent detector for nitric oxide (NO). Essentially nonfluorescent until it reacts with NO to yield the highly fluorescent triazolofluorescein (DAF-2T) (ab145473).

  • Purity

    > 95%
  • CAS Number

    205391-01-1
  • Chemical structure

    Chemical Structure

Properties

  • Excitation

    505nm
  • Emission

    518nm
  • Chemical name

    4,5-Diaminofluorescein
  • Molecular weight

    362.34
  • Molecular formula

    C20H14N2O5
  • Storage instructions

    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in DMSO
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • Source

    Synthetic

References

This product has been referenced in:

  • Sasaki E  et al. Highly sensitive near-infrared fluorescent probes for nitric oxide and their application to isolated organs. J Am Chem Soc 127:3684-5 (2005). Read more (PubMed: 15771488) »
  • Gabe Y  et al. Highly sensitive fluorescence probes for nitric oxide based on boron dipyrromethene chromophore-rational design of potentially useful bioimaging fluorescence probe. J Am Chem Soc 126:3357-67 (2004). Read more (PubMed: 15012166) »
  • Räthel TR  et al. Application of 4,5-diaminofluorescein to reliably measure nitric oxide released from endothelial cells in vitro. Biol Proced Online 5:136-142 (2003). Read more (PubMed: 14569611) »

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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