Key features and details
- Cell permeable reagent for quantification of low concentrations of nitric oxide in solution.
- CAS Number: 254109-22-3
- Purity: > 98%
- Soluble in DMSO
- Form / State: Solid
- Source: Synthetic
Product nameDAF-FM DA, Nitric oxide quantification reagent
DescriptionCell permeable reagent for quantification of low concentrations of nitric oxide in solution.
- 4-Amino-5-methylamino- 2',7'-difluorofluorescein diacetate
- DAF-FM diacetate
- Diaminofluorescein-FM diacetate
Cell permeable reagent for quantification of low concentrations of nitric oxide in solution. Essentially nonfluorescent until it is hydrolyzed to DAF-FM (ab145297) by intracellular esterases and reacts with NO to form a fluorescent benzotriazole.
Chemical name3-Amino-4-(N-methylamino)-2',7'-difluorofluorescein diacetate
Storage instructionsStore at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in DMSO
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab145295 has not yet been referenced specifically in any publications.