Key features and details
- Goat polyclonal to DAGLA
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-DAGLA antibody
DescriptionGoat polyclonal to DAGLA
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
- WB: Human liver lysates; IHC-P: Mouse cerebellum, Human breast, pancreas and spleen tissues; ICC/IF: SKNSH cell line.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab81984 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 115 kDa.
1 hour primary incubation is recommended for this product.
|IHC-P||1/500 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionCatalyzes the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), the most abundant endocannabinoid in tissues. Required for axonal growth during development and for retrograde synaptic signaling at mature synapses.
Tissue specificityHighly expressed in brain and pancreas.
Involvement in diseaseDefects in DAGLA may be a cause of spinocerebellar ataxia type 20 (SCA20) [MIM:608687]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA20 is an autosomal dominant, adult-onset form characterized by dysarthria due to spasmodic dysphonia followed by slowly progressive ataxia. Note=A copy number variation consisting of a 260-kb duplication at chromosome 11q12.2-12.3 is responsible for SCA20. The critical gene within the duplicated segment may be DAGLA.
Sequence similaritiesBelongs to the AB hydrolase superfamily. Lipase family.
Cellular localizationCell membrane.
- Information by UniProt
- C11orf11 antibody
- Chromosome 11 open reading frame 11 antibody
- DAGL(ALPHA) antibody
Anti-DAGLA antibody (ab81984) at 0.3 µg/ml + human liver lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 115 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
Additional bands at: 22 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Some minor background is detected and is blocked by the immunizing peptide.
Immunohistochemistical detection of DAGLA using ab81984 on formaldehyde fixed rat cerebellum tissue sections (cerebellum). Antigen retrieval step: heat mediated in citric acid pH6. 1%BSA as blocking agent for 10 mins@ rt°C. Primary antibody dilution 1/1000 for 2 hours. Secondary antibody: anti-sheep IgG conjugated to biotin (1/200). The pattern of immunolabelling is very similar to that obtained in mouse. However, the cell bodies of some Purkinje cells are also positive, this appears to be punctate and evenly distributed.
Immunohistochemistical detection of DAGLA with ab81984 on formaldehyde fixed mouse cerebellum sections. Antigen retrieval step: heat mediated in citric acid pH6. 1% BSA used as blocking agent for 10 mins @ rt°C. Primary antibody ab81984 incubated at 1/500 for 2 hours. Secondary antibody: anti sheep IgG conjugated to biotin (1/200). The upper image shows DAGL immunostaining with ab81984 on wildtype mouse cerebellum, the lower image shows an absence of DAGL alpha immunostaining on knockout mouse mouse cerebellum. These results are consistent with published data with other anti-DAGL alpha antibodies (http://jcb.rupress.org/cgi/content/full/163/3/463)
ab81984 has been referenced in 4 publications.
- Xue SS et al. Repetitive high-frequency transcranial magnetic stimulation reverses depressive-like behaviors and protein expression at hippocampal synapses in chronic unpredictable stress-treated rats by enhancing endocannabinoid signaling. Pharmacol Biochem Behav 184:172738 (2019). PubMed: 31229467
- Okubo Y et al. Diacylglycerol lipase alpha promotes tumorigenesis in oral cancer by cell-cycle progression. Exp Cell Res 367:112-118 (2018). WB . PubMed: 29614312
- Smaga I et al. Changes in the Brain Endocannabinoid System in Rat Models of Depression. Neurotox Res 31:421-435 (2017). PubMed: 28247204
- Hutch CR et al. An endocannabinoid system is present in the mouse olfactory epithelium but does not modulate olfaction. Neuroscience 300:539-53 (2015). WB ; Mouse . PubMed: 26037800