Key features and details
- Rabbit polyclonal to DAGLB - C-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DAGLB antibody - C-terminal
See all DAGLB primary antibodies
DescriptionRabbit polyclonal to DAGLB - C-terminal
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
- HeLa, 293T and Jurkat whole cell lysates.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab191159 was affinity purified using an epitope specific to DAGLB immobilized on solid support.
Our Abpromise guarantee covers the use of ab191159 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 74 kDa.|
|IP||Use a concentration of 2 - 10 µg/ml.|
FunctionCatalyzes the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), the most abundant endocannabinoid in tissues. Required for axonal growth during development and for retrograde synaptic signaling at mature synapses.
Sequence similaritiesBelongs to the AB hydrolase superfamily. Lipase family.
Cellular localizationCell membrane.
- Information by UniProt
- Daglb antibody
- DAGLBETA antibody
- DGL beta antibody
All lanes : Anti-DAGLB antibody - C-terminal (ab191159) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 74 kDa
Exposure time: 3 minutes
Lysates prepared using NETN lysis buffer.
Detection of DAGLB in immunoprecipitates of 293T whole cell lysate prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab191159 at 6 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection (Lane 1). Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
ab191159 has not yet been referenced specifically in any publications.