Key features and details
- Rabbit polyclonal to DAI
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DAI antibody
DescriptionRabbit polyclonal to DAI
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment (His-tag) corresponding to Human DAI aa 10-167. Expressed in E.coli.
Database link: Q9H171
- WB: Recombinant human DAI; Human serum; Jurkat whole cell lysate (ab7899). IHC-P: Human kidney, liver and colorectal cancer tissue.
This product was previously labelled as Z DNA binding protein
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232977 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 46 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionMay play a role in host defense against tumors and pathogens. Binds Z-DNA.
Tissue specificityHighly expressed in lymphatic tissues including lymph node, leukocytes, tonsil, bone marrow and spleen. Expressed to a lesser extent in thymus, lung and liver.
Sequence similaritiesContains 2 DRADA repeats.
- Information by UniProt
- C20orf183 antibody
- DAI antibody
- DLM 1 antibody
Anti-DAI antibody (ab232977) at 2 µg/ml + Recombinant human DAI.
Predicted band size: 46 kDa
All lanes : Anti-DAI antibody (ab232977) at 2 µg
Lane 1 : Human serum
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/1000 dilution
Predicted band size: 46 kDa
Formalin-fixed, paraffin-embedded human liver tissue stained for DAI using ab232977 at 30 μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for DAI using ab232977 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human kidney tissue stained for DAI using ab232977 at 20 μg/ml in immunohistochemical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232977 has not yet been referenced specifically in any publications.