Anti-DAI antibody (ab232977)
Key features and details
- Rabbit polyclonal to DAI
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DAI antibody -
Description
Rabbit polyclonal to DAI -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment (His-tag) corresponding to Human DAI aa 10-167. Expressed in E.coli.
Database link: Q9H171 -
Positive control
- WB: Recombinant human DAI; Human serum; Jurkat whole cell lysate (ab7899). IHC-P: Human kidney, liver and colorectal cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232977 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 46 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 46 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
May play a role in host defense against tumors and pathogens. Binds Z-DNA. -
Tissue specificity
Highly expressed in lymphatic tissues including lymph node, leukocytes, tonsil, bone marrow and spleen. Expressed to a lesser extent in thymus, lung and liver. -
Sequence similarities
Contains 2 DRADA repeats. - Information by UniProt
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Database links
- Entrez Gene: 81030 Human
- Omim: 606750 Human
- SwissProt: Q9H171 Human
- Unigene: 302123 Human
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Alternative names
- C20orf183 antibody
- DAI antibody
- DLM 1 antibody
see all
Images
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Anti-DAI antibody (ab232977) at 2 µg/ml + Recombinant human DAI.
Predicted band size: 46 kDa -
All lanes : Anti-DAI antibody (ab232977) at 2 µg
Lane 1 : Human serum
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/1000 dilution
Predicted band size: 46 kDa -
Formalin-fixed, paraffin-embedded human liver tissue stained for DAI using ab232977 at 30 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for DAI using ab232977 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human kidney tissue stained for DAI using ab232977 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab232977 has not yet been referenced specifically in any publications.